Molecular mechanism of transforming growth factor (TGF)-beta1-induced glutathione depletion in alveolar epithelial cells. Involvement of AP-1/ARE and Fra-1

The Journal of Biological Chemistry
Hazel JardineI Rahman

Abstract

Glutathione (GSH) is a ubiquitous antioxidant in lung epithelial cells and lung lining fluid. Transforming growth factor beta1 (TGF-beta1) is a pleiotropic cytokine involved in cellular proliferation and differentiation. The level of TGF-beta1 is elevated in many chronic inflammatory lung disorders associated with oxidant/antioxidant imbalance. In this study, we show that TGF-beta1 depletes GSH by down-regulating expression of the enzyme responsible for its formation, gamma-glutamylcysteine synthetase (gamma-GCS) and induces reactive oxygen species production in type II alveolar epithelial cells (A549). To investigate the molecular mechanisms of inhibition of glutathione synthesis, we employed reporters containing fragments from the promoter region of the gamma-GCS heavy subunit (h), the gene that encodes the catalytic subunit of gamma-GCS. We found that TGF-beta1 reduced the expression of the long gamma-GCSh construct (-3802/GCSh-5'-Luc), suggesting that an antioxidant response element (ARE) may be responsible for mediating the TGF-beta1 effect. Interestingly, the electrophoretic mobility shift assay revealed that the DNA binding activity of both activator protein-1 (AP-1) and ARE was increased in TGF-beta1-treated epithelial ...Continue Reading

References

May 29, 1992·Biochemical and Biophysical Research Communications·J J GippR T Mulcahy
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R S FrilingV Daniel
Aug 1, 1991·American Journal of Respiratory Cell and Molecular Biology·N KhalilA H Greenberg
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F J StaalL A Herzenberg
Feb 1, 1989·The American Review of Respiratory Disease·A M CantinR G Crystal
Jul 1, 1987·Journal of Applied Physiology·A M CantinR G Crystal
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Sep 1, 1995·Thorax·W MacNee, I Rahman
Nov 10, 1995·The Journal of Biological Chemistry·K N SchmidtP A Baeuerle
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·K YoshiokaM Karin
Mar 24, 1995·The Journal of Biological Chemistry·T XieA K Jaiswal
Dec 1, 1993·Journal of Applied Physiology·J H RoumR G Crystal
Nov 1, 1993·The American Review of Respiratory Disease·E Bunnell, E R Pacht
Dec 22, 1995·The Journal of Biological Chemistry·V J Thannickal, B L Fanburg
Dec 24, 1996·Biochemical and Biophysical Research Communications·I RahmanC A Smith
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·R Venugopal, A K Jaiswal
May 29, 1997·Biochemical and Biophysical Research Communications·K R SekharM L Freeman
Dec 16, 1998·American Journal of Respiratory and Critical Care Medicine·W I de BoerJ H van Krieken
Aug 12, 1999·Free Radical Biology & Medicine·I RahmanW MacNee
Nov 5, 1999·Biochemical and Biophysical Research Communications·Y Chen, K S Ramos
Nov 24, 1999·The Journal of Biological Chemistry·A C WildR T Mulcahy
Dec 22, 1999·The Journal of Biological Chemistry·D WottonJ Massagué
Dec 22, 1999·The Journal of Biological Chemistry·M E EngelH L Moses
Jul 21, 2000·American Journal of Respiratory and Critical Care Medicine·S I HagiwaraS Kitamura
Oct 12, 2000·The European Respiratory Journal·I Rahman, W MacNee
May 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·E L Dahl, R T Mulcahy
Jun 20, 2001·The Journal of Clinical Investigation·J F PittetD Sheppard

❮ Previous
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Citations

Nov 1, 2003·Biochemical and Biophysical Research Communications·Jadwiga K Kepa, David Ross
Jan 8, 2008·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Xi Huang, Robert B Finkelman
Feb 21, 2006·Antioxidants & Redox Signaling·Hye-Youn ChoSteven R Kleeberger
Nov 6, 2007·Antioxidants & Redox Signaling·Rui-Ming Liu
Sep 26, 2008·Antioxidants & Redox Signaling·Latoya N Johnson, Michael Koval
Feb 1, 2011·Antioxidants & Redox Signaling·Christian E OvergaardMichael Koval
Dec 19, 2009·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Chiao-Nan Joyce ChenLaDora V Thompson
Jul 30, 2004·Molecular and Cellular Biology·C BerruyerF Galland
Aug 12, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carolyn J BagloleRichard P Phipps
Apr 24, 2013·Journal of Cardiothoracic Surgery·Xianfeng QuDongguo Wang
Feb 3, 2016·Journal of Endocrinological Investigation·G LiuY Wang
Jul 22, 2015·Archives of Toxicology·Hye-Youn Cho, Steven R Kleeberger
Jun 17, 2015·Oxidative Medicine and Cellular Longevity·Jelena KrstićJuan F Santibanez
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Sep 2, 2008·Molecular Aspects of Medicine·Saibal K Biswas, Irfan Rahman
Jun 15, 2007·Toxicologic Pathology·Amy YangFernando Ramiro-Ibáñez
May 27, 2005·The Journal of Biological Chemistry·Tomonori HosoyaMasayuki Yamamoto
Sep 13, 2005·Journal of Cellular Physiology·Muyao LiNaomi K Fukagawa
Jan 13, 2006·American Journal of Physiology. Cell Physiology·Yoshihiro TakamuraDhirendra P Singh
Apr 29, 2005·The Journal of Pharmacology and Experimental Therapeutics·Hiroshi AkahoriToshinari Takamura
Jan 5, 2008·The Journal of Pharmacology and Experimental Therapeutics·Anthony Y H WooChristopher H K Cheng
Nov 9, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sekhar P M Reddy, Brooke T Mossman
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher C FranklinNelson Fausto
Nov 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicole SchoenfeldStefan Grimm
Sep 5, 2013·Biological Chemistry·Francisco J Sánchez-GómezSantiago Lamas
Jul 9, 2020·American Journal of Respiratory Cell and Molecular Biology·Kamran AtabaiMichael J Podolsky
Jan 16, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heather MaundersAudrey Richter
Jan 9, 2017·Cardiovascular Drugs and Therapy·Russell G Rogers, Jeffrey S Otis
Jan 31, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carolyn J BagloleRichard P Phipps
Sep 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rui-Ming LiuMargaret M Tarpey
Jun 8, 2012·Annals of the New York Academy of Sciences·Christian E OvergaardMichael Koval
Sep 10, 2017·Redox Biology·Cristina Espinosa-DíezSantiago Lamas
Jan 5, 2005·Free Radical Biology & Medicine·Andrei V BakinMichael L Freeman

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