Molecular mechanisms of 2, 3', 4, 4', 5-pentachlorobiphenyl-induced thyroid dysfunction in FRTL-5 cells

PloS One
Hui YangYu Duan

Abstract

Polychlorinated biphenyls (PCBs) can severely interfere with multiple animals and human systems. To explore the molecular mechanisms underlying 2, 3', 4, 4', 5- pentachlorobiphenyl (PCB118)-induced thyroid dysfunction, Fischer rat thyroid cell line-5(FRTL-5) cells were treated with either different concentrations of PCB118 or dimethyl sulfoxide (DMSO). The effects of PCB118 on FRTL-5 cells viability and apoptosis were assessed by using a Cell Counting Kit-8 assay and apoptosis assays, respectively. Quantitative real-time polymerase chain reaction was used to quantify protein kinase B (Akt), Forkhead box protein O3a (FoxO3a), and sodium/iodide symporter (NIS) mRNA expression levels. Western blotting was used to detect Akt, phospho-Akt (p-Akt), FoxO3a, phospho-FoxO3a (p-FoxO3a), and NIS protein levels. Luciferase reporter gene technology was used to detect the transcriptional activities of FoxO3a and NIS promoters. The effects of the constitutively active Akt (CA-Akt) and dominant-negative Akt (DN-Akt) plasmids on p-Akt, p-FoxO3a, and NIS levels were examined in PCB118-treated FRTL-5 cells. The effects of FoxO3a siRNA on FoxO3a, p-FoxO3a, and NIS protein levels were examined in the PCB118-treated FRTL-5 cells. The effects of pcDN...Continue Reading

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Citations

Nov 1, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Bojin XuYu Duan
Apr 19, 2016·International Journal of Environmental Research and Public Health·Eveline DirinckLuc F Van Gaal
Mar 25, 2018·Endocrine Connections·Bilal B MughalBarbara A Demeneix
Jan 27, 2019·Scientific Reports·Almudena Espín-PérezTheo M C M de Kok
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Sep 9, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Li WangYu Duan
Jan 24, 2016·Reviews in Endocrine & Metabolic Disorders·Leonidas H Duntas, Nikos Stathatos

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Methods Mentioned

BETA
PCR
protein assay
Transfection
Assay
electrophoretic
immunoprecipitation

Software Mentioned

SPSS
McPromoter

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