PMID: 9436535Jan 22, 1998Paper

Molecular mechanisms of erythrophagocytosis. Characterization of the senescent erythrocytes that are phagocytized by macrophages

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
Daniela BratosinJean Montreuil

Abstract

We have recently developed a flow cytometric assay for the quantitation of erythrophagocytosis, using PKH 26-labeled erythrocytes as the target cells. Using this assay we have shown that there is extensive phagocytosis of desialylated erythrocytes. Furthermore, we have demonstrated that it is the densest population of erythrocytes obtained on a self-forming gradient of Percoll that shows the greatest susceptibility to phagocytosis. We designate this population of erythrocytes as fraction X; it is even denser than the fraction 5 found previously. This population of erythrocytes corresponds to zone X previously seen in the dot-plot of the flow cytometric analyses of human erythrocytes. Further scrutiny of this fraction indicates that a) it shows the greatest reactivity with annexin V, which is specific for the detection of phosphatidylserine (PS) exposed on the outer leaflet of the erythrocyte membrane, b) it is the most susceptible to erythrophagocytosis by resident murine peritoneal macrophages, and c) this erythrophagocytosis of PKH 26-labeled erythrocytes can be inhibited by annexin V and by liposomes containing PS. Scanning electron microscopy of fraction X shows two populations of erythrocytes: (A) spheroechinocytes with fi...Continue Reading

References

Oct 1, 1979·British Journal of Haematology·A KnyszynskiE A Rachmilewitz
Jan 1, 1976·American Journal of Hematology·D AminoffN Ibgebrigtsen
Nov 1, 1975·In Vitro·M Rabinovitch, M J DeStefano
Jan 1, 1991·Experimental Gerontology·K A GutowskiD Aminoff
Jan 1, 1990·Critical Reviews in Oncology/hematology·T ShigaK Kon
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T M AllenR A Schlegel
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H U LutzP Arese
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J VaysseD Aminoff
Apr 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·J Jancik, R Schauer
Sep 1, 1983·American Journal of Hematology·B Smedsrøod, D Aminoff
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M M KayV Rudloff
Feb 11, 1994·Journal of Molecular Biology·C PigaultA Brisson
Nov 13, 1996·Biochimica Et Biophysica Acta·P T Srinivasan, J Basu
Jan 1, 1992·Archives of Gerontology and Geriatrics·D AminoffE Supina

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Citations

May 7, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Leila Zarif
Mar 3, 1999·The European Journal of Neuroscience·S M van den EijndeC I De Zeeuw
Apr 16, 2010·The Journal of Biological Chemistry·Shaobin WangMing-Hui Zou
Sep 10, 2010·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Garry J Handelman, Nathan W Levin
Jun 25, 2009·British Journal of Haematology·Latorya A BarberRobert S Franco
Dec 9, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Daniela BratosinJean Montreuil
Aug 13, 2005·Experimental Cell Research·Constance DelabyFrançois Canonne-Hergaux
Dec 25, 2002·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hubertus P A JersmannIan Dransfield
May 26, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Daniela BratosinJean Montreuil
Sep 15, 2017·FEBS Open Bio·Inês B Santarino, Otília V Vieira
Jul 9, 2005·Transplantation·Christopher BurlakMichael A Rees
Jan 5, 2002·In Vitro Cellular & Developmental Biology. Animal·C RousselleX Ronot
Mar 22, 2020·Pharmaceutics·Larisa KolevaElena Sinauridze
Dec 27, 2016·Frontiers in Cellular and Infection Microbiology·Paulo R R TotinoMaria de Fátima Ferreira-da-Cruz
Apr 2, 2008·Toxicology and Applied Pharmacology·Debabrata BiswasTuli Biswas

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