Molecular mechanisms of extracellular adenine nucleotides-mediated inhibition of human Cd4(+) T lymphocytes activation.

Purinergic Signalling
Xavier DuhantJean-Marie Boeynaems

Abstract

We have previously reported that ATPgammaS, a slowly hydrolyzed analog of ATP, inhibits the activation of human CD4(+) T lymphocytes by anti-CD3 and anti-CD28 mAb. In this report we have partially characterized the signaling mechanisms involved in this immunosuppressive effect. ATPgammaS had no inhibitory effect on CD4(+) T-cell activation induced by PMA and anti-CD28, indicating that it acts proximally to the TCR. It had no effect on the calcium rise induced by CD3/CD28 stimulation, but inhibited the phosphorylation of three kinases, ERK2, p38 MAPK and PKB, that play a key role in the activation of T cells. The receptor involved in these actions remains unidentified.

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Citations

Jun 5, 2012·Blood·Laura VitielloAndrea la Sala
Jul 4, 2012·Journal of Leukocyte Biology·Monali ManoharWolfgang G Junger

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Methods Mentioned

BETA
density gradient centrifugation
PMA
ELISA

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