During clathrin-mediated endocytosis (CME), a flat patch of membrane is invaginated and pinched off to release a vesicle into the cytoplasm. In yeast CME, over 60 proteins-including a dynamic actin meshwork-self-assemble to deform the plasma membrane. Several models have been proposed for how actin and other molecules produce the forces necessary to overcome the mechanical barriers of membrane tension and turgor pressure, but the precise mechanisms and a full picture of their interplay are still not clear. In this review, we discuss the evidence for these force production models from a quantitative perspective and propose future directions for experimental and theoretical work that could clarify their various contributions.
Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery
Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASp
Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces
Endocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity
Reconstructing the evolution of the endocytic system: insights from genomics and molecular cell biology
Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway
Mathematical modeling of endocytic actin patch kinetics in fission yeast: disassembly requires release of actin filament fragments
Quantitative analysis of the mechanism of endocytic actin patch assembly and disassembly in fission yeast
Purification of actin from fission yeast Schizosaccharomyces pombe and characterization of functional differences from muscle actin.
Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast.
Distinct roles for F-BAR proteins Cdc15p and Bzz1p in actin polymerization at sites of endocytosis in fission yeast
Actin filament severing by cofilin dismantles actin patches and produces mother filaments for new patches
Local and global analysis of endocytic patch dynamics in fission yeast using a new "temporal superresolution" realignment method
A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats
Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks
Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors
High-speed superresolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches
Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis
Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis
"Essentially, all models are wrong, but some are useful"-a cross-disciplinary agenda for building useful models in cell biology and biophysics
Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features
Quantification of nanoscale forces in lectin-mediated bacterial attachment and uptake into giant liposomes.
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation.
Endocytosis against high turgor pressure is made easier by partial coating and freely rotating base.
Global Identification of ANTH Genes Involved in Rice Pollen Germination and Functional Characterization of a Key Member, OsANTH3.
Investigation of cellular uptake mechanism of functionalised gold nanoparticles into breast cancer using SERS.
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