PMID: 6095289Dec 1, 1984Paper

Molecular mechanisms of manganese mutagenesis

Proceedings of the National Academy of Sciences of the United States of America
W S El-DeiryA G So

Abstract

The mechanism by which DNA polymerase discriminates between complementary and noncomplementary nucleotides for insertion into a primer terminus has been investigated. Apparent kinetic constants for the insertion of dGTP and dATP into the hook polymer d(C)194-d(G)12 with Escherichia coli DNA polymerase I (large fragment) were determined. The results suggest that the high specificity of base selection by DNA polymerase I is achieved by utilization of both Km and Vmax differences between complementary and noncomplementary nucleotides. The molecular basis for the increased error frequency observed with DNA polymerase I in the presence of Mn2+ has also been investigated. Our studies demonstrate that when Mn2+ is substituted for Mg2+, there is a higher ratio of insertion of incorrect to correct dNTP by the polymerase activity, accompanied by a decreased hydrolysis of a mismatched dNMP relative to a matched dNMP at the primer terminus by the 3',5' exonuclease activity. Kinetic analysis revealed that in the presence of Mn2+, the kcat for insertion of a complementary dNTP is reduced, whereas the catalytic rate for the insertion of a mismatched nucleotide is increased. The apparent Km values for either complementary or noncomplementary n...Continue Reading

References

Aug 8, 1977·Biochemical and Biophysical Research Communications·M MiyakiT Ono
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S LinnR Holliday
Sep 28, 1968·Journal of Molecular Biology·Z W Hall, I R Lehman
Dec 1, 1965·Journal of Molecular Biology·A Orgel, L E Orgel
Oct 26, 1967·Biochemical and Biophysical Research Communications·K R HansonE Havir
Jan 1, 1982·Annual Review of Biochemistry·L A Loeb, T A Kunkel
Apr 25, 1983·Journal of Molecular Biology·A R FershtW C Tsui
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M Watanabe, M F Goodman
Dec 16, 1964·Biochimica Et Biophysica Acta·G BERNARDI
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER

❮ Previous
Next ❯

Citations

Jul 1, 1991·Mutation Research·M De MéoG Duménil
Mar 5, 1999·Mutation Research·K A Eckert, P L Opresko
Jul 11, 2002·Neurochemistry International·Alan S Hazell
Mar 31, 1998·Current Opinion in Structural Biology·C A Brautigam, T A Steitz
Aug 14, 1999·Journal of Biomolecular Structure & Dynamics·D BarskyM E Colvin
Oct 20, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Farida Louise AssemLeonard Stephen Levy
Jan 1, 1987·Journal of Toxicology and Environmental Health·B SrisuchartR P Sharma
May 21, 1997·Journal of the National Cancer Institute·M PaoliniG Cantelli-Forti
Nov 19, 2013·Biochemistry. Biokhimii︠a︡·L V GeningV Z Tarantul
Mar 25, 2010·BMC Evolutionary Biology·Carolina Díaz Arenas, Niles Lehman
May 1, 1991·Environmental Health Perspectives·E T Snow
Sep 1, 1994·Environmental Health Perspectives·O S Bhanot, J J Solomon
Jul 23, 2013·PLoS Computational Biology·Joshua I GlaserKonrad P Kording
Aug 29, 2012·PloS One·Bradley Michael ZamftGeorge Church
Jan 7, 2014·PloS One·Carolina Díaz Arenas, Niles Lehman
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·S MoranE T Kool
May 1, 1987·Bulletin of Environmental Contamination and Toxicology·M PaoliniG Bronzetti
Oct 7, 1998·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·A V Koudrine
Feb 18, 2011·Bioengineered Bugs·Mary Abou-Nader, Michael J Benedik
Jan 10, 2017·Genes·Thomas A Guilliam, Aidan J Doherty
Jan 26, 1988·Biochemistry·W S el-DeiryK M Downey
Sep 20, 1988·Biochemistry·T G HecklerS M Hecht
Jan 22, 1991·Biochemistry·S S CarrollS J Benkovic
Mar 23, 2017·Biochemistry. Biokhimii︠a︡·K A ZakharchevaV Z Tarantul
Sep 10, 2020·Chemical Communications : Chem Comm·Tatsuya FunaiHidehito Urata
Jan 1, 1986·Environmental Mutagenesis·J D Roberts, T A Kunkel
May 14, 2016·Physical Review. E·Pierre Gaspard
Jul 5, 2007·The Journal of Biological Chemistry·Ekaterina G Frank, Roger Woodgate
Sep 19, 2020·ELife·Aleksandra BebelPhoebe A Rice

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.