PMID: 45003Oct 18, 1979

Molecular mechanisms of olfactory reception. IV. Some biochemical characteristics of the camphor receptor from rat olfactory epithelium

Biochimica Et Biophysica Acta
E E FesenkoL D Krapivinskaya

Abstract

Some parameters of the receptor element from the rat olfactory epithelium are evaluated; it is characterized by high affinity for camphor (KD = 1.5. x 10(-9) M). Triton X-100 has no marked effect on the binding of [3H]camphor. Neither RNAase nor phospholipase C affected [3H]camphor-binding activity. Pronase and trypsin abolished [3H]camphor binding activity by 65 and 40%, respectively. Sulfhydryl reagents decrease the binding of [3H]camphor by a factor of 5--8. The isoelectric point of the receptor solubilized with Triton X-100 is 4.8, as determined by isoelectric focusing. The molecular weight of the receptor as determined by gel electrophoresis is about 120 000. It is proposed that the camphor receptor is a membrane protein containing sulfhydryl groups and playing a key role in olfactory reception.

References

Jan 1, 1984·Progress in Neurobiology·T V GetchellM L Getchell
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N E BaldacciniA Topazzini
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J PevsnerS H Snyder
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J PevsnerS H Snyder
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R LernerA Roby
Mar 15, 1986·Experientia·G Ohloff
Sep 26, 2007·The FEBS Journal·G B PostnikovaV S Sivozhelezov
Jan 1, 1988·Bioelectromagnetics·T M PhilippovaS I Alekseev
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·P Pelosi
Jun 3, 1985·European Journal of Biochemistry·E BignettiR Tirindelli

Citations

Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M L Getchell, R C Gesteland

Related Concepts

SDS-PAGE
Smell Perception
Sulfhydryl Compounds
Plasma Protein Binding Capacity
Mesothelium
Trypsin
Olfactory Mucosa
Sulfhydryl Reagents
Camphor
Plcb1

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