PMID: 9538506Apr 16, 1998Paper

Molecular mechanisms of polymyxin B-membrane interactions: direct correlation between surface charge density and self-promoted transport

The Journal of Membrane Biology
A WieseU Seydel

Abstract

We have studied the interaction of the polycationic peptide antibiotic polymyxin B (PMB) with asymmetric planar bilayer membranes via electrical measurements. The bilayers were of different compositions, including those of the lipid matrices of the outer membranes of various species of Gram-negative bacteria. One leaflet, representing the bacterial inner leaflet, consisted of a phospholipid mixture (PL; phosphatidylethanolamine, -glycerol, and diphosphatidylglycerol in a molar ratio of 81:17:2). The other (outer) leaflet consisted either of lipopolysaccharide (LPS) from deep rough mutants of PMB-sensitive (Escherichia coli F515) or -resistant strains (Proteus mirabilis R45), glycosphingolipid (GSL-1) from Sphingomonas paucimobilis IAM 12576, or phospholipids (phosphatidylglycerol, diphytanoyl-phosphatidylcholine). In all membrane systems, the addition of PMB to the outer leaflet led to the induction of current fluctuations due to transient membrane lesions. The minimal PMB concentration required for the induction of the lesions and their size correlated with the charge of the lipid molecules. In the membrane system resembling the lipid matrix of a PMB-sensitive strain (F515 LPS/PL), the diameters of the lesions were large enoug...Continue Reading

Citations

Jul 21, 2006·The Journal of Physical Chemistry. B·Kandaswamy VijayanMark Goulian
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·R DaugelaviciusD H Bamford
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·P M FurneriG Tempera
Apr 18, 2002·Antimicrobial Agents and Chemotherapy·B LindnerA Dalhoff
Feb 24, 2006·Antimicrobial Agents and Chemotherapy·Rajalakshmi BalakrishnaSunil A David
Nov 15, 2006·Infection and Immunity·Lisa E MånssonAgneta Richter-Dahlfors
Jun 24, 2005·Infection and Immunity·Katharina KranzerWulf Schneider-Brachert
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Jul 14, 2010·Biochemistry. Biokhimii︠a︡·D S Kabanov, I R Prokhorenko
Sep 24, 1999·Biological Chemistry·A WieseS Müller-Loennies
Jan 29, 2014·Future Medicinal Chemistry·Eliana AlvesAdelaide Almeida
Aug 10, 2007·Expert Opinion on Investigational Drugs·Chanaz Salmi, Jean M Brunel
Nov 25, 2014·Antibiotics·Kathryn L NawrockiShonna M McBride
Mar 3, 2009·Proteomics·María Fernández-ReyesLuis Rivas
Oct 11, 2008·Chemical Biology & Drug Design·Ponnurengam Malliappan SivakumarMukesh Doble
Feb 16, 2011·Biophysical Journal·Jakubs KubiakLuis A Bagatolli
Aug 24, 2004·Journal of Molecular Biology·Jefferson D Knight, Andrew D Miranker
Apr 14, 2011·Pharmaceutical Development and Technology·Kenneth S BrandenburgHayat Önyüksel
Aug 31, 2016·Pharmaceutical Development and Technology·Yang LiLi Yang
Jun 3, 2005·The Journal of Biological Chemistry·Emilisa FrirdichChris Whitfield

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