PMID: 9658077Jul 11, 1998Paper

Molecular mechanisms of serum resistance of human influenza H3N2 virus and their involvement in virus adaptation in a new host

Journal of Virology
M MatrosovichY Kawaoka

Abstract

H3N2 human influenza viruses that are resistant to horse, pig, or rabbit serum possess unique amino acid mutations in their hemagglutinin (HA) protein. To determine the molecular mechanisms of this resistance, we characterized the receptor-binding properties of these mutants by measuring their affinity for total serum protein inhibitors and for soluble receptor analogs. Pig serum-resistant variants displayed a markedly decreased affinity for total pig serum sialylglycoproteins (which contain predominantly 2-6 linkage between sialic acid and galactose residues) and for the sialyloligosaccharide 6'-sialyl(N-acetyllactosamine). These properties correlated with the substitution 186S-->I in HA1. The major inhibitory activity in rabbit serum was found to be a beta inhibitor with characteristics of mannose-binding lectins. Rabbit serum-resistant variants exhibited decreased sensitivity to this inhibitor due to the loss of a glycosylation sequon at positions 246 to 248 of the HA. In addition to a somewhat reduced affinity for 6'-sialyl(N-acetyllactosamine)-containing receptors, horse serum-resistant variants lost the ability to bind the viral neuraminidase-resistant 4-O-acetylated sialic acid moieties of equine alpha2-macroglobulin bec...Continue Reading

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Citations

Mar 21, 2008·Journal of Virology·Jeffrey MeisnerDavid A Steinhauer
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Colin R ParrishPeter Daszak
Apr 8, 2010·Biochemistry. Biokhimii︠a︡·E M RapoportN V Bovin
Dec 22, 2009·Influenza and Other Respiratory Viruses·Irina KiselevaLarisa Rudenko
Sep 3, 2011·Influenza and Other Respiratory Viruses·Kevin T CwachVictor C Huber
Mar 11, 2003·Reviews in Medical Virology·Mikhail Matrosovich, Hans-Dieter Klenk
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y HaD C Wiley
Dec 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma R JobPatrick C Reading
Sep 13, 2005·Annual Review of Microbiology·Colin R Parrish, Yoshihiro Kawaoka
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Sep 6, 2003·Biological Chemistry·Matthias IwersenRoland Schauer
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Erik de VriesCornelis A M de Haan
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick C ReadingAlberto Mantovani
Nov 6, 2013·FEMS Microbiology Reviews·Wander Van BreedamHans J Nauwynck
Apr 21, 2021·Trends in Microbiology·Louisa E WallaceCornelis A M de Haan
May 3, 2002·Reviews in Medical Virology·Ralf WagnerHans-Dieter Klenk

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