Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases

Frontiers in Genetics
Kozo Tomita, Seisuke Yamashita


The universal 3'-terminal CCA sequence of tRNA is built and/or synthesized by the CCA-adding enzyme, CTP:(ATP) tRNA nucleotidyltransferase. This RNA polymerase has no nucleic acid template, but faithfully synthesizes the defined CCA sequence on the 3'-terminus of tRNA at one time, using CTP and ATP as substrates. The mystery of CCA-addition without a nucleic acid template by unique RNA polymerases has long fascinated researchers in the field of RNA enzymology. In this review, the mechanisms of RNA polymerization by the remarkable CCA-adding enzyme and its related enzymes are presented, based on their structural features.


Sep 4, 2014·International Journal of Molecular Sciences·Kozo Tomita
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Oct 9, 2014·Frontiers in Genetics·Akio Kanai
Sep 24, 2016·RNA Biology·Christiane Rammelt, Walter Rossmanith
Jun 8, 2017·Nature Communications·Seisuke YamashitaKozo Tomita
Aug 23, 2019·Journal of Molecular Evolution·George H Jones
May 31, 2020·International Journal of Molecular Sciences·Angelo SladeMartin Holcik
Oct 28, 2017·Scientific Reports·Matus ValachGertraud Burger
Aug 22, 2018·Frontiers in Genetics·Kozo Tomita, Yining Liu
Nov 30, 2018·Frontiers in Genetics·Yuka Yashiro, Kozo Tomita
Dec 3, 2020·International Journal of Molecular Sciences·Oliver HennigMario Mörl

Related Concepts

Adenosine Triphosphate
Nucleic Acids
Research Personnel
DNA-Directed RNA Polymerase
Transfer RNA
TRNA adenylyltransferase
TRNA 3'-terminal CCA Addition
Enzymatic Biochemistry

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