Molecular mechanisms underlying FOXP3 induction in human T cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Pierre-Yves MantelCarsten B Schmidt-Weber

Abstract

FOXP3 is playing an essential role for T regulatory cells and is involved in the molecular mechanisms controlling immune tolerance. Although the biological relevance of this transcription factor is well documented, the pathways responsible for its induction are still unclear. The current study reveals structure and function of the human FOXP3 promoter, revealing essential molecular mechanisms of its induction. The FOXP3 promoter was defined by RACE, cloned, and functionally analyzed using reporter-gene constructs in primary human T cells. The analysis revealed the basal, T cell-specific promoter with a TATA and CAAT box 6000 bp upstream the translation start site. The basal promoter contains six NF-AT and AP-1 binding sites, which are positively regulating the trans activation of the FOXP3 promoter after triggering of the TCR. The chromatin region containing the FOXP3 promoter was bound by NF-ATc2 under these conditions. Furthermore, FOXP3 expression was observed following TCR engagement. Promoter activity, mRNA, and protein expression of T cells were suppressed by addition of cyclosporin A. Taken together, this study reveals the structure of the human FOXP3 promoter and provides new insights in mechanisms of addressing T regul...Continue Reading

References

Mar 26, 1981·Nature·C Benoist, P Chambon
Oct 20, 1995·The Journal of Biological Chemistry·G HagenG Suske
Apr 1, 1994·The Journal of Allergy and Clinical Immunology·J M CarballidoK Blaser
Jun 1, 1993·Immunological Reviews·J F Miller, W R Heath
Oct 31, 1996·Nature·A K AbbasA Sher
Nov 26, 1999·Gene·G Suske
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M Thornton, E M Shevach
Mar 29, 2001·Molecular Immunology·C B Schmidt-WeberA H Lichtman
Jun 8, 2001·The Journal of Experimental Medicine·E M Shevach
Aug 3, 2001·The Journal of Biological Chemistry·L A SchubertS F Ziegler
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R KhattriF Ramsdell
Dec 12, 2001·Nature Immunology·Marc A GavinAlexander Rudensky
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazumi AsaiShuichi Kaminogawa
Jan 11, 2003·Science·Shohei HoriShimon Sakaguchi
Jan 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S KunzmannC B Schmidt-Weber
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 4, 2003·Nature Immunology·Roli KhattriFred Ramsdell
Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jan G WohlfahrtCarsten B Schmidt-Weber
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tse-Ching ChenHerman Waldmann
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Magdalena J PolanczykHalina Offner
Sep 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Shuang FuJonathan S Bromberg
Dec 4, 2004·The Journal of Allergy and Clinical Immunology·Christian KaragiannidisCarsten B Schmidt-Weber
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patricia E RaoPaul D Ponath
Mar 5, 2005·Blood·Manuela BattagliaMaria-Grazia Roncarolo
Mar 23, 2005·Immunity·Jason D FontenotAlexander Y Rudensky
Mar 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Estelle BettelliMohamed Oukka

❮ Previous
Next ❯

Citations

Aug 12, 2006·Springer Seminars in Immunopathology·Troy R Torgerson
Dec 22, 2006·European Journal of Immunology·Steven F Ziegler
Feb 9, 2007·Journal of the American Society of Nephrology : JASN·Marina NorisGiuseppe Remuzzi
Sep 15, 2007·Journal of Virology·Shuo LiUlf Dittmer
Sep 12, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K G HaanstraM Jonker
Feb 21, 2008·PloS One·Peter C J JansonOla Winqvist
Aug 24, 2007·Transplantation·Elaine Long, Kathryn J Wood
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina OberleElisabeth Suri-Payer
Dec 8, 2007·Annual Review of Immunology·Thomas R Malek
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephan SauerMatthias Merkenschlager
Mar 8, 2008·PloS One·Zoran Popmihajlov, Kendall A Smith
Dec 9, 2008·Current Opinion in Organ Transplantation·Pervinder SagooRobert I Lechler
Apr 7, 2009·British Journal of Haematology·Kazuhiro NakamuraMasao Kobayashi
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Girdhari LalJonathan S Bromberg
Aug 22, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Sonia Y VelásquezCristiam M Alvarez
Apr 28, 2009·Transplantation·Oriol BestardJosep M Grinyó
Jul 31, 2009·Blood·Girdhari Lal, Jonathan S Bromberg
Jun 22, 2010·Journal of Cellular and Molecular Medicine·Lorna S JohnstoneMarie A Dziadek
Jun 25, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Julia K PolanskyJochen Huehn
Jun 18, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timothy J SadlonSimon C Barry
Nov 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin LeiMasaki Yasukawa
Aug 18, 2010·Blood·Harriet A PurvisCatharien M U Hilkens
Sep 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Noelia CasaresJuan José Lasarte
Apr 25, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seong Wook KangInsoo Kang
Sep 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Martin VaethFriederike Berberich-Siebelt
Sep 18, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deirdre M FullerWeiguo Zhang
Dec 25, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Julien ZuberYvon Lebranchu
Jul 28, 2013·International Reviews of Immunology·Veerle Fleskens, Ruben van Boxtel
Jan 15, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Koji SugimotoShinichi Matsumoto
Dec 4, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chihiro OgawaMasahide Tone
Jul 23, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Xiaoning ZhangChunhong Ma
Apr 3, 2014·Diabetes/metabolism Research and Reviews·L Reinert-HartwallUNKNOWN DIABIMMUNE Study Group
Apr 10, 2014·Immunological Reviews·Xiaomei YuanThomas R Malek
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chaoran LiQi-Jing Li
Aug 16, 2014·Immunological Reviews·Subhash K Tripathi, Riitta Lahesmaa
Aug 19, 2014·Journal of Immunology Research·Reiko Takahashi, Akihiko Yoshimura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.