Molecular methods for the diagnosis and characterization of Cryptococcus: a review

Canadian Journal of Microbiology
José Júlio Costa SidrimMarcos Fábio Gadelha Rocha

Abstract

Cryptococcosis is a fungal infection caused by yeasts of the genus Cryptococcus, with Cryptococcus neoformans and Cryptococcus gattii as the primary pathogenic species. This disease is a threat to immunocompromised patients, especially those who have AIDS. However, the disease has also been described in healthy individuals. The tests used to identify these microorganisms have limitations that make final diagnosis difficult. However, currently there are specific gene sequences that can be used to detect C. neoformans and C. gattii from clinical specimens and cultures. These sequences can be used for identification, typing, and the study of population genetics. Among the main identification techniques are hybridization, which was the pioneer in molecular identification and development of specific probes for pathogen detection; PCR and other PCR-based methods, particularly nested PCR and multiplex PCR; and sequencing of specific genomic regions that are amplified through PCR, which is especially useful for diagnosis of cryptococcosis caused by unconventional Cryptococcus sp. Concerning microorganism typing, the following techniques have shown the best ability to differentiate between fungal serotypes and molecular types: PCR finge...Continue Reading

References

May 1, 1992·Molecular Ecology·H HadrysB Schierwater
May 1, 1992·Journal of Clinical Microbiology·E D Spitzer, S G Spitzer
Nov 1, 1992·Journal of Clinical Microbiology·A Varma, K J Kwon-Chung
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Dec 1, 1989·Journal of General Microbiology·A Varma, K J Kwon-Chung
Jan 1, 1989·Journal of General Microbiology·I Polacheck, G A Lebens
Oct 1, 1989·Journal of Bacteriology·B I Restrepo, A G Barbour
Jun 1, 1987·Biochemical Society Transactions·A J Jeffreys
Jul 1, 1995·Journal of Clinical Microbiology·A VarmaK J Kwon-Chung
Feb 1, 1994·PCR Methods and Applications·M C Edwards, R A Gibbs
Feb 1, 1993·Journal of Clinical Microbiology·K E Huffnagle, R M Gander
Nov 1, 1995·Journal of Clinical Microbiology·G S SandhuG D Roberts
Nov 1, 1996·Letters in Applied Microbiology·P RumaA G Brownlee
Sep 23, 1997·BioTechniques·O HenegariuP H Vogt
Feb 17, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·S C ChenT C Sorrell
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M C MaidenB G Spratt
Oct 17, 1998·Journal of Clinical Microbiology·P RappelliA Aceti
Sep 11, 1999·Trends in Ecology & Evolution·U G Mueller, L L Wolfenbarger
Sep 17, 1999·Journal of Clinical Microbiology·D Garcia-HermosoF Dromer
Dec 22, 1999·Trends in Microbiology·M C Enright, B G Spratt
Jan 20, 2000·Infection and Immunity·G M CoxJ R Perfect
Apr 3, 2001·Microbiology·T BoekhoutW Meyer
May 29, 2001·Journal of Clinical Microbiology·M P HayetteP De Mol
Oct 3, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R KanoA Hasegawa
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·Ralf BialekUdo Reischl
Aug 1, 2002·Journal of Clinical Microbiology·Guizhen Luo, Thomas G Mitchell
Feb 27, 2003·Emerging Infectious Diseases·Wieland MeyerUNKNOWN IberoAmerican Cryptococcal Study Group
Apr 22, 2003·Infection and Immunity·P T MageeDana Davis
May 16, 2003·FEMS Yeast Research·Agnes Kiesling CasaliMarilene Henning Vainstein

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Citations

Aug 13, 2011·Journal of Clinical Microbiology·Sara GagoMaría J Buitrago
Sep 24, 2011·Medical Mycology·Julie HarrisTom Chiller
Jun 3, 2014·BMC Microbiology·Erin J KelleyDavid M Engelthaler
Sep 15, 2015·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Vanessa RiveraTonny Naranjo
Nov 26, 2015·Journal of Clinical Pathology·Adepemi O OgundejiOlihile M Sebolai
Oct 16, 2012·Journal of Medical Microbiology·Manjula M WeerasekeraRichard D Cannon
Oct 4, 2014·Clinical Microbiology Reviews·Sharon C-A ChenTania C Sorrell
May 4, 2011·Chembiochem : a European Journal of Chemical Biology·Gianfranco De PascaleGerard D Wright
Oct 3, 2017·Biomédica : revista del Instituto Nacional de Salud·Sunny SánchezMaría Teresa Illnait-Zaragozí
Sep 26, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Laura J SchmertmannMark B Krockenberger
Sep 24, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Wan Ling ChengTze Ping Loh
Apr 8, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Reza Kamali KakhkiKiarash Ghazvini
Aug 7, 2019·Journal of Fungi·Justin BeardsleySharon C-A Chen
Mar 30, 2021·Revista iberoamericana de micología·Joycenea da Silva MatsudaFlor Ernestina Martinez-Espinosa
May 25, 2021·Frontiers in Cellular and Infection Microbiology·Nan HongJianping Xu
Jul 27, 2021·Frontiers in Medicine·Marc A Judson

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