Molecular modifications in factor VIII concentrates produced from different plasma pools

Thrombosis Research
E L SaenkoH Schwinn

Abstract

We defined the main cause for the increased immunogenicity of the commercial factor VIII (fVIII) plasma-derived concentrates reported to induce formation of inhibitory antibodies in haemophilia A patients in Germany and Belgium. Formation of these antibodies directed against the C2 domain of fVIII was previously attributed to the use of solvent/detergent (S/D) treatment and pasteurisation for virus inactivation of fVIII concentrate. Since fVIII concentrates associated with increased immunogenicity were prepared from plasma pools characterised by elevated levels of coagulation markers, we examined whether the plasma source or S/D treatment and pasteurisation are responsible for structural changes within the C2 domain causing its abnormal immunogenicity. We found that samples of fVIII concentrate that originated from the abnormal plasma pool had a reduced ability to bind to phospholipid and conformationally sensitive anti-C2 domain antibodies, this effect being mostly pronounced in the samples that underwent both S/D treatment and pasteurisation. Thus, our study suggests that insufficient quality of the starting plasma pools is the major factor determining the structural alterations in the C2 domain of fVIII, whereas combination ...Continue Reading

References

Jun 14, 1977·Biochemistry·Y BarenholzR D Carlson
Jan 1, 1989·Haemostasis·R J WagenvoordH C Hemker
Jan 1, 1983·Vox Sanguinis·R Pflugshaupt, G Kurt
Jun 9, 1995·The Journal of Biological Chemistry·E L Saenko, D Scandella
Jan 1, 1995·Advances in Experimental Medicine and Biology·L W Hoyer

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Citations

May 13, 2008·Blood·Sébastien Lacroix-DesmazesSuryasarathi Dasgupta
Jun 25, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·J AstermarkM T Reding
May 13, 2006·Seminars in Hematology·Jan Astermark

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