Molecular multi-effect screening of environmental pollutants using the MolDarT

Environmental Toxicology
Anja LiedtkeRik I L Eggen

Abstract

Molecular effect detection is a useful approach for ecotoxicological screening of chemicals. We show here the application of the molecular DarT (MolDarT), where the expression of selected target genes is detected in short-term (120 h) exposed developing zebrafish (Danio rerio), thus allowing subacute multi-effect compound screening. The genes metallothionein 2 (mt2), cytochrome P450 1A1 (cyp1a1), and recombination activation gene 1 (rag1) are used as endpoints that describe detoxification/metal toxicity (mt2), detoxification/PAH toxicity (cyp1a1), and acquired immune system disruption (rag1). Each gene's developmental expression was studied in unexposed zebrafish during 4 to 120 h past fertilization (hpf), and all three genes were found to be expressed at 120 hpf. Furthermore, mt2 transcripts were present at high levels at 4 hpf, indicating a maternal transfer. For positive toxicity controls, freshly fertilized zebrafish eggs were exposed for 120 hpf to ZnSO(4), 1,5-dimethylnaphthalene (DMN) and CdCl(2). Exposure to 100 and 200 microM ZnSO(4) significantly induced mt2; 10 microM DMN and 20 microM DMN resulted in significantly increased cyp1a1 abundance; and 5 and 10 microM CdCl(2) significantly reduced rag1 expression levels. F...Continue Reading

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Citations

Apr 30, 2013·Aquatic Toxicology·Katalin BakosBalázs Kovács
Aug 9, 2011·Aquatic Toxicology·Derek Alsop, Chris M Wood
Apr 8, 2014·Bulletin of Environmental Contamination and Toxicology·Debdip Mukhopadhyay, Ansuman Chattopadhyay
Aug 8, 2015·Environmental Toxicology and Pharmacology·Debdip MukhopadhyayAnsuman Chattopadhyay

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