Molecular mutation profile of Pfcrt and Pfmdr1 in Plasmodium falciparum isolates from Bioko Island, Equatorial Guinea

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Jian LiZongyun Chen

Abstract

Antimalarial drug resistance is a primary public health problem. Haplotypes of pfcrt and pfmdr1 gene have been implicated to be molecular markers of chloroquine (CQ) resistance. This study aims to explore mutation distribution of Pfcrt and Pfmdr1 in Bioko Island, Equatorial Guinea (EG). Blood samples were collected from different districts of Bioko. The single nucleotide polymorphisms in Pfcrt (codons 72 to 76) and Pfmdr1 (codons 86, 130, 184, 1034, 1042, 1109 and 1246) were assessed by nested PCR with DNA sequencing and haplotype prevalences were also determined. Analysis of Pfcrt and Pfmdr1 mutations was successful in 151 and 157 samples respectively out of the 172 samples taken for this study. The mutations of Pfcrt and Pfmdr1 were found in 98.67% and 89.81% isolates, respectively. The Pfcrt 74-76, Pfmdr1 86, and Pfmdr1 184 were 92.05%, 50.32%, and 87.26% found mostly of mutation type, respectively. Three haplotypes coding 72-76 of Pfcrt were found including CVMNK, CVIET, and CVM/I N/E K/T, which accounted for 1.33%, 92.05%, and 6.62%, respectively. No mutation in Pfmdr1-N1 codon at 130 and Pfmdr1-N2 (S1034C, N1042D, V1109I, and D1246Y) was detected. The types coding 86 and 184 in Pfmdr1 were found including NY, YY, NF, YF, ...Continue Reading

References

Oct 2, 1965·Lancet·T HarinasutaC Viravan
Feb 15, 2001·The New England Journal of Medicine·A DjimdéC V Plowe
Feb 15, 2001·The New England Journal of Medicine·D C Warhurst
Jan 16, 2004·Lancet·M AdjuikUNKNOWN International Artemisinin Study Group
Feb 11, 2010·Antimicrobial Agents and Chemotherapy·Sean GriffingVenkatachalam Udhayakumar
Sep 2, 2011·The Journal of Infectious Diseases·Frederick N Baliraine, Philip J Rosenthal
Nov 14, 2012·Parasites & Vectors·Hans J OvergaardMichel A Slotman
May 9, 2013·Malaria Journal·Andrea M RehmanImmo Kleinschmidt
May 23, 2014·The Lancet Infectious Diseases·Zhao-Rong LunLin-Chun Fu
Sep 25, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·D P Mawili-MboumbaM K Bouyou Akotet
Jan 20, 2015·Nature Genetics·Olivo MiottoDominic P Kwiatkowski

❮ Previous
Next ❯

Citations

Mar 19, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Narayani Prasad KarAparup Das
Apr 14, 2017·Malaria Journal·Diana Gómez-BarrosoAgustín Benito
May 4, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Mariangela L'EpiscopiaCarlo Severini
Oct 2, 2021·Frontiers in Cellular and Infection Microbiology·Xi HeZhaoqing Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.