Molecular nature of mutations induced by high-LET irradiation with argon and carbon ions in Arabidopsis thaliana

Mutation Research
Tomonari HiranoTomoko Abe

Abstract

Linear energy transfer (LET) is an important parameter to be considered in heavy-ion mutagenesis. However, in plants, no quantitative data are available on the molecular nature of the mutations induced with high-LET radiation above 101-124keVμm(-1). In this study, we irradiated dry seeds of Arabidopsis thaliana with Ar and C ions with an LET of 290keVμm(-1). We analyzed the DNA alterations caused by the higher-LET radiation. Mutants were identified from the M(2) pools. In total, 14 and 13 mutated genes, including bin2, egy1, gl1, gl2, hy1, hy3-5, ttg1, and var2, were identified in the plants derived from Ar- and C-ions irradiation, respectively. In the mutants from both irradiations, deletion was the most frequent type of mutation; 13 of the 14 mutated genes from the Ar ion-irradiated plants and 11 of the 13 mutated genes from the C ion-irradiated plants harbored deletions. Analysis of junction regions generated by the 2 types of irradiation suggested that alternative non-homologous end-joining was the predominant pathway of repair of break points. Among the deletions, the proportion of large deletions (>100bp) was about 54% for Ar-ion irradiation and about 64% for C-ion irradiation. Both current results and previously reported...Continue Reading

References

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Nov 17, 2011·BMC Plant Biology·Yusuke KazamaTomoko Abe

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Citations

Dec 24, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Yan DuLibin Zhou
Feb 19, 2015·The Plant Journal : for Cell and Molecular Biology·Tomonari HiranoTomoko Abe
Apr 15, 2016·Bioscience, Biotechnology, and Biochemistry·Koji YamadaKengo Suzuki
Sep 13, 2013·Genes & Genetic Systems·Yusuke KazamaTomoko Abe
Jul 28, 2016·Genes & Genetic Systems·Kotaro IshiiTomoko Abe
Jul 26, 2017·Letters in Applied Microbiology·W HuJ Chen
Aug 18, 2018·Dose-response : a Publication of International Hormesis Society·Miaomiao ZhangDong Lu
Feb 25, 2021·Bioscience, Biotechnology, and Biochemistry·Kazuhide TsuneizumiTomoko Abe

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