Molecular networks affected by neonatal microbial colonization in porcine jejunum, luminally perfused with enterotoxigenic Escherichia coli, F4ac fimbria or Lactobacillus amylovorus

PloS One
P TrevisiP Bosi

Abstract

The development of an early complex gut microbiota may play an important role in the protection against intestinal dysbiosis later in life. The significance of the developed microbiota for gut barrier functionality upon interaction with pathogenic or beneficial bacteria is largely unknown. The transcriptome of differently perfused jejunal loops of 12 caesarian-derived pigs, neonatally associated with microbiota of different complexity, was studied. Piglets received pasteurized sow colostrum at birth (d0), a starter microbiota (Lactobacillus amylovorus (LAM), Clostridium glycolicum, and Parabacteroides) on d1-d3, and a placebo inoculant (simple association, SA) or an inoculant consisting of sow's diluted feces (complex association, CA) on d3-d4. On d 26-37, jejunal loops were perfused for 8 h with either enterotoxigenic Escherichia coli F4 (ETEC), purified F4 fimbriae, LAM or saline control (CTRL). Gene expression of each intestinal loop was analyzed by Affymetrix Porcine Gene 1.1_ST array strips. Gene Set Enrichment Analysis was performed on expression values. Compared to CTRL, 184 and 74; 2 and 139; 2 and 48 gene sets, were up- and down-regulated by ETEC, F4 and LAM, respectively. ETEC up-regulated networks related to inflamma...Continue Reading

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Citations

May 10, 2019·Animals : an Open Access Journal From MDPI·Micol BertocchiPaolo Trevisi
Mar 20, 2020·Journal of Animal Science and Biotechnology·Paolo TrevisiPaolo Bosi
Feb 9, 2019·Journal of Animal Physiology and Animal Nutrition·Diana LuisePaolo Trevisi
May 23, 2020·Animals : an Open Access Journal From MDPI·Diana LuisePaolo Trevisi
Feb 7, 2021·Microorganisms·Paolo TrevisiPaolo Bosi
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Federico CorreaPaolo Trevisi
Jul 24, 2021·Applied Microbiology and Biotechnology·A CheccucciPaolo Trevisi
Dec 8, 2021·Scientific Reports·M Saladrigas-GarcíaS M Martín-Orúe

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Datasets Mentioned

BETA
GSE77787

Methods Mentioned

BETA
PCR

Software Mentioned

Affymetrix Expression Console
Cytoscape
LAM
SAS
Ensembl
Transcriptome Analysis Consolle
Gene Set Enrichment Analysis

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