Molecular order and dynamics in bilayers consisting of highly polyunsaturated phospholipids

Biophysical Journal
Drake C Mitchell, Burton J Litman

Abstract

The time-resolved fluorescence emission and decay of fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to characterize equilibrium and dynamic bilayer structural properties of symmetrically substituted phosphatidylcholines (PCs) with acyl chains containing no, one, four, or six double bonds and mixed-chain phosphatidylcholines with a saturated sn-1 chain and one, four, or six double bonds in the sn-2 chain. Both the Brownian rotational diffusion (BRD) model and the wobble-in-cone model were fit to all differential polarization data, and the descriptions of the data provided by the BRD model were found to be statistically superior. Global analysis of differential polarization data revealed two statistically equivalent solutions. The solution corresponding to a bimodal orientational distribution function, f(theta), was selected based on the effects of temperature on f(theta) and previous measurements on fixed, oriented bilayers. The overall equilibrium acyl chain order in these bilayers was analyzed by comparing the orientational probability distribution for DPH, f(theta) sin theta, with a random orientational distribution. Orientational order decreased and probe dynamics increased in mixed-chain species as ...Continue Reading

References

Dec 1, 1977·Biophysical Journal·K KinositaA Ikegami
Jan 1, 1992·Methods in Enzymology·M L Johnson, L M Faunt
Apr 1, 1987·Biophysical Journal·J R AlcalaF G Prendergast
Sep 1, 1981·Journal of Biochemical and Biophysical Methods·J R LakowiczA Balter
Dec 12, 1993·Biochimica Et Biophysica Acta·J Hernandez-Borrell, K M Keough
Aug 1, 1997·Biophysical Journal·D HusterK Gawrisch

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Citations

Feb 3, 2005·European Biophysics Journal : EBJ·Marja T Hyvönen, Petri T Kovanen
Oct 12, 2005·European Biophysics Journal : EBJ·Miguel A R B Castanho, Miguel X Fernandes
Jul 10, 2003·Lipids·Drake C MitchellBurton J Litman
May 25, 2012·Cell Biochemistry and Biophysics·Paola A CorsettoAngela M Rizzo
May 15, 2003·Archives of Biochemistry and Biophysics·Victoria T ArmstrongWilliam Stillwell
Sep 26, 2003·Biophysical Journal·Howard L BrockmanJohn A Glomset
Oct 29, 2003·Chemistry and Physics of Lipids·William Stillwell, Stephen R Wassall
Apr 25, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T GueckH Fuhrmann
May 22, 2008·The British Journal of Nutrition·Robert S ChapkinJoanne R Lupton
Nov 2, 2005·Molecular and Cellular Biology·Christopher E EllisRobert L Nussbaum
Mar 18, 2008·Journal of Lipid Research·Kathleen Boesze-BattagliaSteven J Fliesler
Aug 13, 2009·European Journal of Pediatrics·Jan Philipp SchuchardtAndreas Hahn
Nov 9, 2007·Journal of Pharmaceutical Sciences·Célia SousaSalette Reis
Mar 26, 2009·Pharmacology & Therapeutics·Renata GorjãoRui Curi
Nov 14, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Klaus GawrischMihaela Mihailescu
Apr 29, 2008·Experimental Cell Research·Nelson B ColeRobert L Nussbaum
Aug 8, 2007·Journal of the Neurological Sciences·Sabrina Florent-BéchardThierry Oster
Apr 20, 2011·Journal of Molecular Recognition : JMR·Nikolay BuzhynskyySimon Scheuring
Feb 19, 2010·Molecular Nutrition & Food Research·Jeffrey AtkinsonJohn Katsaras
Jun 28, 2005·Biophysical Journal·Shui-Lin Niu, Drake C Mitchell
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Seung-Cheol LeeRichard D Ludescher
Apr 25, 2015·Trends in Cell Biology·Bruno AntonnyThierry Ferreira
Jan 10, 2006·Biophysical Journal·Mikko HeikeläMarja T Hyvönen
Feb 22, 2005·Biophysical Journal·Jarmila RepákováIlpo Vattulainen
Feb 14, 2012·Biophysical Journal·Mozhgan NazariHeiko Heerklotz
Mar 13, 2012·Chemistry and Physics of Lipids·Drake C MitchellBurton J Litman
Oct 9, 2004·Biochimica Et Biophysica Acta·H Raghuraman, Amitabha Chattopadhyay
Aug 1, 2016·Biochimica Et Biophysica Acta·António M T M do CantoLuís M S Loura
Nov 9, 2016·Frontiers in Physiology·Stefan Gahbauer, Rainer A Böckmann
May 5, 2005·American Journal of Epidemiology·Minyi LuPaul F Jacques
Feb 1, 2017·The Journal of Physical Chemistry. B·Carla M RosettiClaudio Pastorino
Jun 29, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luís M S Loura, J P Prates Ramalho

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