Molecular ordering of cellulose after extraction of polysaccharides from primary cell walls of Arabidopsis thaliana: a solid-state CP/MAS (13)C NMR study

Carbohydrate Research
Lynette M DaviesRoger H Newman

Abstract

Solid-state CP/MAS 13C NMR spectroscopy was used to determine the effects of three different sequential extraction procedures, used to remove non-cellulosic polysaccharides, on the molecular ordering of cellulose in a cell-wall preparation containing mostly primary cell walls obtained from the leaves of the model dicotyledon, Arabidopsis thaliana. The extractions were 50 mM trans-1,2-diaminocyclohexane N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate (giving Residue 1); 50 mM CDTA, 50 mM sodium carbonate and 1 M KOH (giving Residue 2); and 50 mM CDTA, 50 mM sodium carbonate and 4 M KOH (giving Residue 3). The molecular ordering of cellulose in Residue 1 was similar to that in unextracted walls: the cellulose was almost all crystalline, with 43% of molecules contained in crystallite interiors and similar proportions of the triclinic (I(alpha)) and monoclinic (I(beta)) crystal forms. Residue 2 was partly decrystallized and the remaining crystallites were mostly in the I(beta) form. Residue 3 was a mixture of cellulose II, cellulose I and amorphous cellulose. The presence of signals at 100.0 and 102.3 ppm in the spectra of Residues 1 and 2, but not of unextracted cell walls, suggested that the extractions giving these ...Continue Reading

References

Apr 1, 1995·Plant Physiology·E ZablackisP Albersheim
Jul 21, 2000·Solid State Nuclear Magnetic Resonance·R H Newman

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Citations

Jul 2, 2009·Journal of Structural Biology·Julian C ThimmLaurence D Melton
Sep 27, 2003·Phytochemical Analysis : PCA
Apr 10, 2015·Magnetic Resonance in Chemistry : MRC·Heather L WheelerAndre Simpson
Aug 25, 2020·Advanced Materials·Eleni ToumpanakiStephen J Eichhorn
Jun 5, 2003·Planta·Lynette M Davies, Philip J Harris
Jul 13, 2004·Biomacromolecules·Adriana SturcováMichael C Jarvis

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