Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg

Journal of Bacteriology
Hideyuki OhshimaYoshito Sadaie

Abstract

Transcriptional analysis and disruption of five open reading frames (ORFs), ydiO, ydiP, ydiR, ydiS, and ydjA, in the prophage 3 region of the chromosome of Bacillus subtilis Marburg revealed that they are component genes of the intrinsic BsuM restriction and modification system of this organism. The classical mutant strain RM125, which lacks the restriction and modification system of B. subtilis Marburg, lacks the prophage 3 region carrying these five ORFs. These ORFs constitute two operons, the ydiO-ydiP operon and the ydiR-ydiS-ydjA operon, both of which are expressed during the logarithmic phase of growth. The predicted gene products YdiO and YdiP are the orthologues of cytosine DNA methyltransferases. The predicted YdiS product is an orthologue of restriction nucleases, while the predicted YdiR and YdjA products have no apparent paralogues and orthologues whose functions are known. Disruption of the ydiR-ydiS-ydjA operon resulted in enhanced transformation by plasmid DNA carrying multiple BsuM target sequences. Disruption of ydiO or ydiP function requires disruption of at least one of the following genes on the chromosome: ydiR, ydiS, and ydjA. The degrees of methylation of the BsuM target sequences on chromosomal DNAs were...Continue Reading

References

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Citations

Mar 20, 2003·FEMS Microbiology Letters·Kei AsaiYoshito Sadaie
Jul 24, 2012·Nucleic Acids Research·Yoshikazu Furuta, Ichizo Kobayashi
Nov 23, 2006·Journal of Bacteriology·Ashlee M EarlRoberto Kolter
Jan 29, 2008·Journal of Bacteriology·Kei AsaiYoshito Sadaie
Nov 4, 2005·Journal of Bacteriology·Nora AuCharles M Lovett
Feb 18, 2010·PloS One·Hongjun DongYin Li
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Naoto OhtaniMitsuhiro Itaya
Jun 1, 2011·Microbial Biotechnology·Hein TripOscar P Kuipers
Jan 8, 2008·FEMS Microbiology Letters·Yoshito SadaieKei Asai
Mar 16, 2005·FEMS Microbiology Letters·Satoshi MatsuokaYoshito Sadaie
Dec 23, 2006·Microbiology·Kei AsaiYoshito Sadaie
Nov 22, 2020·Nature Communications·Yiming NiuJoshua S Chappie
Jul 12, 2020·Journal of Structural Biology·Christopher J HosfordJoshua S Chappie

Related Concepts

Endodeoxyribonuclease BsuM
GroESL protein, Bacteria
Natto Bacteria
Bacterial Proteins
Chromosomes, Bacterial
DNA, Bacterial
Genes, Bacterial
Operon
Episomes
Transcription, Genetic

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