Molecular organization of Mason-Pfizer monkey virus capsids assembled from Gag polyprotein in Escherichia coli

Journal of Virology
M V NermutEric Hunter

Abstract

We describe the results of a study by electron microscopy and image processing of Gag protein shells-immature capsids--of Mason-Pfizer monkey virus assembled in Escherichia coli from two truncated forms of the Gag precursor: Deltap4Gag, in which the C-terminal p4Gag was deleted, and Pro(-)CA.NC, in which the N-terminal peptides and proline 1 of the CA domain were deleted. Negative staining of capsids revealed small patches of holes forming a trigonal or hexagonal pattern most clearly visible on occasional tubular forms. The center-to-center spacing of holes in the network was 7.1 nm in Deltap4Gag capsids and 7.4 nm in Pro(-)CA.NC capsids. Image processing of Deltap4Gag tubes revealed a hexagonal network of holes formed by six subunits with a single subunit shared between rings. This organization suggests that the six subunits are contributed by three trimers of the truncated Gag precursor. Similar molecular organization was observed in negatively stained Pro(-)CA.NC capsids. Shadowed replicas of freeze-etched capsids produced by either construct confirmed the presence of a hexagonal network of holes with a similar center-to-center spacing. We conclude that the basic building block of the cage-like network is a trimer of the Del...Continue Reading

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Citations

Dec 9, 2003·Journal of Molecular Biology·Narayanasamy NandhagopalVolker M Vogt
Dec 11, 2002·Journal of Molecular Biology·Keith MayoEric Barklis
Apr 15, 2003·Journal of Virology·Uta K von SchwedlerWesley I Sundquist
Apr 15, 2008·Current Opinion in Structural Biology·Barbie K Ganser-PornillosWesley I Sundquist
Jan 16, 2007·Micron : the International Research and Review Journal for Microscopy·Milan V Nermut, Barbara Mulloy
Jun 2, 2009·Journal of Molecular Biology·Jirí VlachRichard Hrabal
Mar 7, 2014·Journal of Virology·Di L BushVolker M Vogt
Jun 9, 2020·FEBS Letters·Filip KaufmanMichaela Rumlová

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