PMID: 2496981Apr 15, 1989Paper

Molecular organization of RNP complexes containing P11 antigen in heat-shocked and non-heat-shocked Drosophila cells

European Journal of Biochemistry
C SchuldtE K Bautz

Abstract

Immunofluorescence analysis of polytene chromosomes of Drosophila melanogaster using the monoclonal antibody P11 has shown that after heat-shock the 38-kDa P11 antigen almost exclusively localizes at heat-shock puff 93D where it is part of giant puff-specific RNP granules. The biochemical experiments reported here show that, independent of growth temperature, the P11 antigen is a component of nuclear 10S RNP particles. The P11-containing 10S snRNPs can be stabilized in CsCl with 20 mM Mg2+ and possess a buoyant density of rho = 1.4 g/cm3. Sucrose gradient analysis of nuclear RNP extracts of heat-shocked Schneider's S-3 tissue culture cells shows that, after a 37 degree C heat-shock, the 10S RNPs associate with large RNP complexes sedimenting at 170-220S. The change in distribution is a temperature-dependent process with intermediate forms at 29 degrees C and 33 degrees C. In thermotolerant cells this observed change in distribution is strongly reduced. DEAE-Sephacel column chromatography and sucrose gradient analysis of nuclear RNP, followed by Northern blot analysis using 93D-specific probes of the TaqI repeat and immunoblotting experiments, show that the P11-containing 10S snRNPs are distinct from the RNP complexes formed by ...Continue Reading

References

Jun 1, 1979·Cell·M Ashburner, J J Bonner
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1986·Annual Review of Genetics·M R Green
Jun 5, 1986·Journal of Molecular Biology·M N Lelay-TahaP Jeanteur
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C Garbe, M L Pardue
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Dec 25, 1984·Journal of Molecular Biology·A AlonsoT Schmidt
Sep 25, 1983·Journal of Molecular Biology·A AlonsoE Spiess
Nov 11, 1983·Journal of Immunological Methods·H H GuldnerF A Bautz
Oct 1, 1982·European Journal of Biochemistry·P M KloetzelJ Sommerville

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.