Molecular packing of cord factor and its interaction with phosphatidylinositol in mixed monolayers

Biophysical Journal
R Almog, C A Mannella

Abstract

Cord factor (trehalose 6,6'-dimycolate, CF) is a glycolipid located in the outer mycobacterial cell wall that is implicated in the pathogenesis of mycobacteria. Furthermore, CF is a convenient model for studying mycolic acid residues, the major lipid constituents of the mycobacterial cell wall that are believed to form a barrier against drug penetration. The surface properties of CF and its interactions with phosphatidylinositol (PI) have been investigated using the monolayer technique. During compression/expansion/recompression cycles, CF monolayers switch from a loosely packed to a more tightly packed structure. The change in surface properties suggests a molecular rearrangement, perhaps involving interdigitation of long and short chains of the CF molecules. In CF-PI monolayers, maximal lateral packing density occurs between 0.5 and 0.7 mole fraction CF, which is close to the relative composition of mycolic acid residues and shorter-chain lipids in the mycobacterial cell wall. Low concentrations of CF increase the order in PI monolayers, consistent with CF toxicity involving rigidification of cell membranes.

References

Jan 1, 1978·Progress in the Chemistry of Fats and Other Lipids·C Asselineau, J Asselineau
Jan 2, 1979·European Journal of Biochemistry·E DurandJ F Tocanne
May 1, 1991·Research in Microbiology·G Lanéelle, M Daffé
Jul 1, 1989·Journal of General Microbiology·J L Woodbury, W W Barrow
Jan 1, 1988·Archives of Biochemistry and Biophysics·R AlmogD S Berns
Aug 1, 1988·Biochimie·N Rastogi, H L David
Apr 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·H L DavidM F Thorel
Dec 22, 1986·Biochimica Et Biophysica Acta·C Huang, J T Mason
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
Aug 24, 1994·Biochimica Et Biophysica Acta·L M CroweJ H Crowe
Jun 1, 1993·Molecular Microbiology·H NikaidoE Y Rosenberg

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Citations

Mar 28, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Christopher W HarlandRaghuveer Parthasarathy
Aug 18, 2000·Immunology and Cell Biology·E R Rhoades, H J Ullrich
Mar 28, 2008·Cellular Microbiology·Sofia AxelrodUlrich E Schaible
Dec 24, 2013·Chemistry and Physics of Lipids·Wilma GroenewaldAnna K Croft
Dec 30, 2014·Frontiers in Cellular and Infection Microbiology·Ainhoa ArbuesCatherine Astarie-Dequeker
Apr 28, 2016·Infectious Diseases·Cheol Moon, Ji-Chan Jang
Oct 30, 2009·Physical Chemistry Chemical Physics : PCCP·Melanie RöefzaadIzabella Brand

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