Molecular phenotype of zebrafish ovarian follicle by serial analysis of gene expression and proteomic profiling, and comparison with the transcriptomes of other animals

BMC Genomics
Anja Knoll-GellidaPatrick J Babin

Abstract

The ability of an oocyte to develop into a viable embryo depends on the accumulation of specific maternal information and molecules, such as RNAs and proteins. A serial analysis of gene expression (SAGE) was carried out in parallel with proteomic analysis on fully-grown ovarian follicles from zebrafish (Danio rerio). The data obtained were compared with ovary/follicle/egg molecular phenotypes of other animals, published or available in public sequence databases. Sequencing of 27,486 SAGE tags identified 11,399 different ones, including 3,329 tags with an occurrence superior to one. Fifty-eight genes were expressed at over 0.15% of the total population and represented 17.34% of the mRNA population identified. The three most expressed transcripts were a rhamnose-binding lectin, beta-actin 2, and a transcribed locus similar to the H2B histone family. Comparison with the large-scale expressed sequence tags sequencing approach revealed highly expressed transcripts that were not previously known to be expressed at high levels in fish ovaries, like the short-sized polarized metallothionein 2 transcript. A higher sensitivity for the detection of transcripts with a characterized maternal genetic contribution was also demonstrated compar...Continue Reading

Associated Datasets

Mar 17, 2006·Patrick J BabinMichele Andre

References

Jan 1, 1992·Molecular Reproduction and Development·K V KandrorA S Stepanov
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z Wang, D D Brown
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S L DeutscherJ D Keene
Jan 1, 1985·The International Journal of Biochemistry·P A Naumoff, P M Stevenson
Mar 1, 1983·Developmental Biology·M R IyengarR M Schultz
Oct 20, 1995·Science·V E VelculescuK W Kinzler
Jun 1, 1993·Molecular and Cellular Biology·D DingH D Lipshitz
Jan 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S OhsakoY Hayashi
Aug 1, 1993·Nature Genetics·M S BoguskiC M Tolstoshev
Jul 1, 1995·Molecular Reproduction and Development·P SchüsslerW Kunz
Jan 1, 1996·Cancer Investigation·P K Tripathi, S K Chatterjee
Jan 1, 1997·Cellular and Molecular Life Sciences : CMLS·P H KroneZ Lele
Aug 22, 1997·The Journal of Biological Chemistry·V G LelianovaY A Ushkaryov
Oct 6, 1997·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·S WelleM Krym
May 14, 1999·Current Opinion in Microbiology·S PhadtareM Inouye
Oct 27, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A K BraatD Zivkovic
Nov 24, 1999·Biochimica Et Biophysica Acta·M HosonoK Nitta
Dec 22, 1999·Molecular Reproduction and Development·A Kanamori
Feb 9, 2000·Genomics·L NeilsonD P Green
Jul 19, 2000·Genome Research·A E LashS F Altschul
Nov 22, 2000·The Journal of Biological Chemistry·R G BootJ M Aerts
Nov 30, 2000·Nucleic Acids Research·K MatsumotoM Tsujimoto
Mar 10, 2001·Developmental Biology·N Matova, L Cooley
Jun 1, 2001·Molecular Human Reproduction·J L Stanton, D P Green
Jun 23, 2001·Biology of Reproduction·C P LeoA J Hsueh
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·R KollmarA J Hudspeth
Dec 4, 2001·Mechanisms of Development·T DickmeisU Strähle
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jun 5, 2002·Molecular and Cellular Endocrinology·J L StantonD P L Green
Sep 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jianjun ChenSan Ming Wang
Sep 17, 2002·Current Opinion in Cell Biology·Shoichiro Tsukita, Mikio Furuse

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Citations

Jan 14, 2011·Fish Physiology and Biochemistry·Ksenia J GrohMarc J-F Suter
Feb 19, 2013·Journal of Proteome Research·Benjamin J ReadingCraig V Sullivan
Jan 24, 2008·Molecular & Cellular Proteomics : MCP·Margaret B LucittTilo Grosser
May 26, 2011·BMC Developmental Biology·Liselotte VesterlundJuha Kere
Feb 6, 2014·Journal of Proteome Research·Justin SchillingBenjamin J Reading
Jan 2, 2014·Omics : a Journal of Integrative Biology·Xi QianGuofang Zhong
Jun 2, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Fabien PierronJean-Charles Massabuau
Jul 18, 2015·International Journal of Molecular Sciences·Weiwei LuoYeke Wang
Apr 23, 2015·Ontogenez·A A Minin, S G Ozerova
Nov 8, 2011·Ecotoxicology and Environmental Safety·Christopher J MartyniukNancy D Denslow
Nov 25, 2016·Bioscience, Biotechnology, and Biochemistry·Jianwu ShiYijiang Hong
Jun 22, 2017·Biochemical and Biophysical Research Communications·Chunmei GeAqin Chen
Apr 21, 2018·Molecular Omics·Xunian ZhouStephen Kwok-Wing Tsui
Apr 25, 2009·Proteomics·Séverine A DegrelleNeil C Talbot
Dec 3, 2009·Proteomics·Marco F L LemosAna C Esteves
Dec 3, 2009·Proteomics·Ignasi FornéJoan Cerdà
May 9, 2008·Biology of Reproduction·Liraz LeviEsther Lubzens
Jul 28, 2013·Marine Biotechnology·Carlos Frederico Ceccon LanesIgor Babiak
Sep 26, 2020·Archives of Toxicology·Alex C KingArmin K Zenker
Jul 18, 2015·Fish Physiology and Biochemistry·Yair Y KohnP Mark Lokman
Mar 11, 2009·General and Comparative Endocrinology·Julien Bobe, Catherine Labbé
Jun 10, 2009·General and Comparative Endocrinology·Esther LubzensJoan Cerdà
May 27, 2011·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jae-Sung RheeJae-Seong Lee

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Datasets Mentioned

BETA
GSE3679
MGC68553
GSM726

Methods Mentioned

BETA
electrophoresis
cDNA library construction
PCR
antisense oligonucleotide

Software Mentioned

UniGene Build
PERL
SAGEmap
Pep
Mascot
Miner
Ensembl
FISHTAG
BLAST
UniGene

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