Molecular phylogenetic analysis of non-sexually transmitted strains of Haemophilus ducreyi

PloS One
Jordan R GastonTricia L Humphreys

Abstract

Haemophilus ducreyi, the etiologic agent of chancroid, has been previously reported to show genetic variance in several key virulence factors, placing strains of the bacterium into two genetically distinct classes. Recent studies done in yaws-endemic areas of the South Pacific have shown that H. ducreyi is also a major cause of cutaneous limb ulcers (CLU) that are not sexually transmitted. To genetically assess CLU strains relative to the previously described class I, class II phylogenetic hierarchy, we examined nucleotide sequence diversity at 11 H. ducreyi loci, including virulence and housekeeping genes, which encompass approximately 1% of the H. ducreyi genome. Sequences for all 11 loci indicated that strains collected from leg ulcers exhibit DNA sequences homologous to class I strains of H. ducreyi. However, sequences for 3 loci, including a hemoglobin receptor (hgbA), serum resistance protein (dsrA), and a collagen adhesin (ncaA) contained informative amounts of variation. Phylogenetic analyses suggest that these non-sexually transmitted strains of H. ducreyi comprise a sub-clonal population within class I strains of H. ducreyi. Molecular dating suggests that CLU strains are the most recently developed, having diverged ap...Continue Reading

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Citations

Dec 15, 2015·Current Opinion in Infectious Diseases·David A Lewis, Oriol Mitjà
Sep 18, 2015·International Journal of STD & AIDS·Sébastien FouéréMichel Janier
Jun 10, 2015·Infection and Immunity·Concerta L HolleyStanley M Spinola
Jul 7, 2015·PLoS Neglected Tropical Diseases·Dharanesh GangaiahStanley M Spinola

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Methods Mentioned

BETA
PCR
electrophoresis
genotyping

Software Mentioned

Sequencher
ImageJ
MEGA
MEGA6

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