Molecular phylogeny and bioprospecting of Endolichenic Fungi (ELF) inhabiting in the lichens collected from a mangrove ecosystem in Sri Lanka

PloS One
Kasun MadurangaPriyani Ashoka Paranagama

Abstract

Endolichenic fungi (ELF) are unexplored group of organisms as a source for the production of bioactive secondary metabolites with radical scavenging activity, antilipase and amylase inhibitory activities. Endolichenic fungi in lichens collected from mangrove or mangrove associated plants are least known for their fungal diversity and potential to produce bioactive compounds. A total of 171 ELF strains were isolated from the lichens collected from mangrove and mangrove associated plants in Puttalam lagoon. Out of this collection, 70 isolates were identified using rDNA-ITS region sequence homology to the GenBank accessions and a phylogenetic analysis was performed. Commonly isolated genera of ELF from lichens were Aspergillus, Byssochlamys, Talaromyces, Diaporthe, Phomopsis, Endomelanconiopsis, Schizophyllum, Cerrena, Trichoderma, Xylaria, Hypoxylon, Daldinia, Preussia, Sordaria, Neurospora, and Lasiodiplodia. In the present study, the effectiveness of ethyl acetate extracts of the ELF isolates were investigated against antioxidant activity, antilipase activity and α-amylase inhibition activity in in-vitro conditions. The results revealed that the extracts of Daldinia eschscholtzii, Diaporthe musigena and Sordaria sp. had the hig...Continue Reading

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Citations

Nov 26, 2019·Planta medica·Meenavalli Babu Govinda RajuluTrichur Subramanian Suryanarayanan
Jul 22, 2019·Microorganisms·Merry Sailonga FaluaburuTakeshi Naganuma

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Methods Mentioned

BETA
Internal Transcribed Spacer
electrophoresis
PCR
PCA
antilipase assay

Software Mentioned

Prism
Neighbor
Graph Pad Prism (
BioNJ
BioEdit
GBlocks
Multiple Sequence Comparison by Log - Expectation ( MUSCLE )
MEGA
Minitab
Basic Local Alignment Search Tool ( BLAST )

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