Molecular Physiology of an Extra-renal Cl(-) Uptake Mechanism for Body Fluid Cl(-) Homeostasis

International Journal of Biological Sciences
Yi-Fang WangPung-Pung Hwang

Abstract

The development of an ion regulatory mechanism for body fluid homeostasis was an important trait for vertebrates during the evolution from aquatic to terrestrial life. The homeostatic mechanism of Cl(-) in aquatic fish appears to be similar to that of terrestrial vertebrates; however, the mechanism in non-mammalian vertebrates is poorly understood. Unlike in mammals, in which the kidney plays a central role, in most fish species, the gill is responsible for the maintenance of Cl(-) homeostasis via Cl(-) transport uptake mechanisms. Previous studies in zebrafish identified Na(+)-Cl(-) cotransporter (NCC) 2b-expressing cells in the gills and skin as the major ionocytes responsible for Cl(-) uptake, similar to distal convoluted tubular cells in mammalian kidney. However, the mechanism by which basolateral ions exit from NCC cells is still unclear. Of the in situ hybridization signals of twelve members of the clc Cl(-) channel family, only that of clc-2c exhibited an ionocyte pattern in the gill and embryonic skin. Double in situ hybridization/immunocytochemistry confirmed colocalization of apical NCC2b with basolateral CLC-2c. Acclimation to a low Cl(-) environment increased mRNA expression of both clc-2c and ncc2b, and also the p...Continue Reading

Citations

Apr 5, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Souichirou TakabeSusumu Hyodo
Aug 25, 2016·General and Comparative Endocrinology·Ying-Jey Guh, Pung-Pung Hwang
Jan 31, 2018·The Journal of Experimental Biology·Michael Brannen, Kathleen M Gilmour
Dec 23, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yoshio TakeiMakoto Kusakabe
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Jun 17, 2016·Proceedings. Biological Sciences·Yi-Fang WangPung-Pung Hwang
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Jun 27, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jason P BrevesAndre P Seale

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Methods Mentioned

BETA
electrophoresis
PCR
in vitro transcription
antisense oligonucleotide

Software Mentioned

Image
Ensembl Genome Browser
BLAST
ClustalW
Ensembl
Pro Plus
Mega

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