Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia

Veterinary Medicine and Science
Souhir SallemiM Gharbi

Abstract

The present study aimed to estimate the molecular prevalence ofTheileria annulataandTrypanosoma evansiinfection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated.T. annulataandT. evansiprevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval (CI) of 95%, while co-infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence ofT. annulatainfection, whereas, there was no significant association shown with the age of cattle andT. evansiinfection. Sequence and phylogenetic analyses showed that theT. annulataTams1 gene andT. evansiITS1 rDNA gene were highly conserved with 97.1-100% and 98.3-100% sequence identity, respectively.

References

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Sep 29, 2009·Tropical Animal Health and Production·Lachhman D SinglaNarinder S Sharma
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Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Feb 6, 2015·Parasite : Journal De La Société Française De Parasitologie·Mohamed Ridha RjeibiMohamed Gharbi

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Datasets Mentioned

BETA
KU145624
KU145623
HQ593643

Methods Mentioned

BETA
PCR
DNA
electrophoresis
glycosylation

Software Mentioned

Epi Info
MEGA
Chromas Pro
BLAST
SPSS

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