Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

Nature Neuroscience
Yue JiangHiroaki Matsunami

Abstract

The mammalian olfactory system uses a large family of odorant receptors (ORs) to detect and discriminate amongst a myriad of volatile odor molecules. Understanding odor coding requires comprehensive mapping between ORs and corresponding odors. We developed a means of high-throughput in vivo identification of OR repertoires responding to odorants using phosphorylated ribosome immunoprecipitation of mRNA from olfactory epithelium of odor-stimulated mice followed by RNA-Seq. This approach screened the endogenously expressed ORs against an odor in one set of experiments using awake and freely behaving mice. In combination with validations in a heterologous system, we identified sets of ORs for two odorants, acetophenone and 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), encompassing 69 OR-odorant pairs. We also identified shared amino acid residues specific to the acetophenone or TMT receptors and developed models to predict receptor activation by acetophenone. Our results provide a method for understanding the combinatorial coding of odors in vivo.

References

Jan 31, 1998·Science·H ZhaoS Firestein
Mar 25, 1999·Cell·B MalnicL B Buck
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·K TouharaT Haga
Dec 2, 2000·Nature Neuroscience·R C AranedaS Firestein
Jan 22, 2002·Nature Neuroscience·Xinmin Zhang, Stuart Firestein
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Aug 13, 2005·Neuroscience and Biobehavioral Reviews·Lorey K TakahashiKendra Watanabe
Sep 18, 2007·Nature·Andreas KellerHiroaki Matsunami
Nov 2, 2007·PLoS Biology·Idan MenasheDoron Lancet
Nov 9, 2007·Nature·Ko KobayakawaHitoshi Sakano
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 6, 2009·Science Signaling·Harumi SaitoJoel D Mainland
Nov 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xavier GrosmaitreMinghong Ma
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Dec 3, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ayumi Nagashima, Kazushige Touhara
Jun 24, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kiyomitsu NaraLinda B Buck
Sep 23, 2011·Nature·Yoh IsogaiCatherine Dulac
Mar 27, 2012·Journal of Neurochemistry·Jingyi LiCharles W Luetje
Aug 29, 2012·Molecular and Cellular Neurosciences·Jingji ZhangThomas Bozza
Nov 28, 2012·Cell·Zachary A KnightJeffrey M Friedman
Oct 19, 2013·ELife·Sean Michael BoyleAnandasankar Ray
Dec 10, 2013·Nature Neuroscience·Joel D MainlandHiroaki Matsunami
Feb 7, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John W ScottKai Zhao
Apr 16, 2014·The Journal of General Physiology·Zita PeterlinMatthew E Rogers
Nov 21, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Timothy S McClintockHiroaki Matsunami
Sep 1, 2015·Nature Neuroscience·Benoît von der WeidIvan Rodriguez

❮ Previous
Next ❯

Citations

Dec 4, 2015·International Journal of Molecular Sciences·Zhenshan WangRunlin Z Ma
Aug 23, 2016·Neurotoxicology·Ramona LehmannChristoph van Thriel
Dec 14, 2017·Scientific Reports·Marta Sanmartí-EspinalJosep Samitier
May 1, 2019·Nature Neuroscience·Gisella VeterePaul W Frankland
Sep 27, 2018·Chemical Senses·Julia MohrhardtMarc Spehr
Nov 6, 2018·Nature Communications·Hitoshi KidaHiroaki Matsunami
Dec 17, 2019·PloS One·Sachiko KoyamaAnthony Mescher
Mar 1, 2020·International Journal of Molecular Sciences·Han Kyoung Choe, Jun Cho
May 20, 2020·Chemical Senses·Timothy S McClintockPatrick Breheny
Sep 1, 2015·Nature Neuroscience·Benoît von der WeidIvan Rodriguez
Sep 26, 2015·Nature Neuroscience·Hirofumi Nishizumi, Hitoshi Sakano
Oct 31, 2019·Chemical Senses·Shashank ChepurwarNitin Gupta
Apr 24, 2020·Genes, Brain, and Behavior·Nicole M Baran, J Todd Streelman
Sep 15, 2018·Science·Kevin A Bolding, Kevin M Franks
May 30, 2019·Chemical Senses·Bilal MalikMehmet Hakan Ozdener
Mar 9, 2017·Frontiers in Genetics·Andrea Degl'Innocenti, Anna D'Errico
Jul 10, 2020·Annual Review of Neuroscience·David H Brann, Sandeep Robert Datta
Jun 2, 2018·Scientific Reports·Lucia SilvottiRoberto Tirindelli
May 26, 2018·Chemical Senses·Longzhi Tan, Xiaoliang Sunney Xie
Oct 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jessie HuangSteven S An
Nov 25, 2020·Annual Review of Physiology·Kensaku Mori, Hitoshi Sakano
Jan 8, 2021·Cell and Tissue Research·Smija M KurianLuis R Saraiva
Aug 28, 2020·Cell Reports·Vinicius Miessler de Andrade CarvalhoFabio Papes
Jan 6, 2017·Journal of Agricultural and Food Chemistry·Lucia M Armelin-Correa, Bettina Malnic
Nov 21, 2018·Journal of Agricultural and Food Chemistry·Eric Block

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE59324

Methods Mentioned

BETA
immunoprecipitation
transgenic
RNA-Seq
PCA
dissection
PCR

Software Mentioned

glmnet
DESeq2
DESeq
WebLogo
ImageJ
R
Bowtie
EdgeR
Clustalx
BEDTools

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.