Molecular Profiling of Pooled Circulating Tumor Cells from Prostate Cancer Patients Using a Dual-Antibody-Functionalized Microfluidic Device

Analytical Chemistry
Changqing YinFubing Wang

Abstract

To capture both epithelial and mesenchymal subpopulations of CTCs at different metastatic stages of PCa patients, here we constructed a novel dual-antibody-functionalized microfluidic device by employing antibodies against PSMA and EpCAM. In vitro experiments with the dual capture system for capturing both LnCAP and LnCAP-EMT cells have shown significantly enhanced capture efficiency as compared to that of the EpCAM single capture system. Furthermore, the dual capture system could successfully identify CTCs in 20 out of 24 (83.3%) PCa patients, and the CTCs counts from the dual capture system were statistically correlated with the TNM stage of patients ( P < 0.05), while conventional diagnostic methods, such as serum PSA level and Gleason score, failed to correlate to patient TNM stages. To further explore potential clinical application of our dual capture system, captured CTCs were recovered and subjected to qRT-PCR to quantify known factors involved in PCa development and therapy. The results demonstrated that the combined detection of SChLAP1 and PSA in CTCs is a potential marker for identifying patients with metastatic PCa, while detection of AR and PD-L1 in CTCs may have the potential to determine the sensitivity of PCa pa...Continue Reading

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Citations

Apr 10, 2019·Nature Reviews. Urology·Kevin M KooMatt Trau
Dec 2, 2020·Cancers·Peng LiuMichiel Stevens
May 4, 2021·Urologic Oncology·Darryl Ethan BernsteinNikhil Vasdev
Jun 5, 2021·Lab on a Chip·K PatersonM Zagnoni
Oct 15, 2020·ACS Combinatorial Science·Dayu HuXiaoyu Cui

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