PMID: 6413654Nov 1, 1983Paper

Molecular properties of Drosophila acetylcholinesterase

Journal of Neurochemistry
S ZingdeK S Krishnan

Abstract

Two distinct classes of acetylcholinesterase (AChE) from the fruit fly Drosophila melanogaster are reported: a soluble species that shows heterogeneity of forms and a particulate species. The subunit composition of the particulate enzyme was studied using the active site label [3H]diisopropylfluorophosphate. Comparison of the electrophoretic patterns on nondenaturing gels using the activity stain and the active site label shows that the label is specific to AChE. The smallest active site-containing subunit of the enzyme is a monomer of approximately 60,000 daltons MW. Two such units are linked by disulphide bonds to produce a dimer of about 110,000 daltons. Another monomeric form of MW approximately 64,000 daltons, although present, does not participate in the dimerisation. The particulate enzyme when solubilised exists as a 9-10S species as determined by sucrose gradient centrifugation. This species has a MW greater than 200,000, as shown by its behaviour on a coarse-bead Sephadex-G200 column. Electrophoretic analysis suggests a MW of nearly 250,000 daltons for this form. Thus, this species is likely to be a tetramer. One possibility is that this tetramer is made up of two units of 64,000 daltons each and a dimer of 110,000 da...Continue Reading

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S BonJ Massoulié
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Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

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Citations

Nov 1, 1987·Journal of Neuroimmunology·N RaneK S Krishnan
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·S W MelansonL Pezzementi
Jan 1, 1988·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·A M Burnell, N P Wilkins
Feb 1, 1989·Developmental Biology·W J Wolfgang, M A Forte
Jan 1, 1991·Parasitology·I D HarrowN A Evans
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H SoreqP Spierer
Feb 1, 1987·Analytical Biochemistry·J Manjrekar, K S Krishnan

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