Molecular properties of gp100-reactive T-cell receptors drive the cytokine profile and antitumor efficacy of transgenic host T cells

Pigment Cell & Melanoma Research
Jonathan M EbyI Caroline Le Poole

Abstract

To study the contribution of T-cell receptors (TCR) to resulting T-cell responses, we studied three different human αβ TCRs, reactive to the same gp100-derived peptide presented in the context of HLA-A*0201. When expressed in primary CD8 T cells, all receptors elicited classic antigen-induced IFN-γ responses, which correlated with TCR affinity for peptide-MHC in the order T4H2 > R6C12 > SILv44. However, SILv44 elicited superior IL-17A release. Importantly, in vivo, SILv44-transgenic T cells mediated superior antitumor responses to 888-A2 + human melanoma tumor cells upon adoptive transfer into tumor-challenged mice while maintaining IL-17 expression. Modeling of the TCR ternary complexes suggested architectural differences between SILv44 and the other complexes, providing a potential structural basis for the observed differences. Overall, the data reveal a more prominent role for the T-cell receptor in defining host T-cell physiology than traditionally assumed, while parameters beyond IFN-γ secretion and TCR affinity ultimately determine the reactivity of tumor-reactive T cells.

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Citations

Feb 23, 2020·American Journal of Respiratory Cell and Molecular Biology·Fei HanI Caroline Le Poole
Jan 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Angela R SmithBrian M Baker
Jan 21, 2021·Physical Chemistry Chemical Physics : PCCP·A C L PereiraU L Fulco
Sep 1, 2019·The Journal of Investigative Dermatology·Emilia R DellaceccaI Caroline Le Poole
Mar 7, 2021·Cancers·Prathyaya RameshI Caroline Le Poole

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