PMID: 3763958Aug 1, 1986Paper

Molecular properties of solubilized CCK receptor from guinea-pig pancreas

Regulatory Peptides
A ZahidiA Ribet

Abstract

In order to characterize the CCK receptor in guinea-pig pancreas, iodinated CCK-39 was bound to pancreatic membranes and the reversible complex was solubilized using various non-denaturing detergents. In term of recovery of ligand stabilized receptors, the relative potencies were Zwittergent 3-14 greater than CHAPS = CHAPSO greater than digitonin greater than MEGA 10 greater than octyl beta-D-glucopyranoside. The stability of receptor complexes was increased by glycerol. Chromatographic analysis revealed that digitonin was the most efficient detergent for disaggregation of CCK receptor complex since it yielded a 76 kDa component in addition to the large components obtained after solubilization with CHAPS and Zwittergent. Furthermore, CCK receptors were covalently labelled using dissuccinimidyl suberate or UV irradiation of labelled membranes by photoactivable radioiodinated CCK-39 and subsequently solubilized by CHAPS + SDS or by SDS alone. A predominant molecule was characterized by chromatography (76 kDa) and SDS-PAGE (89 kDa). In addition to this component, other components having molecular masses of 130-150 kDa, 57 kDa and 40 kDa were detected by SDS-PAGE. They correspond to minor bands. These bands, except the 40 kDa band,...Continue Reading

References

Mar 1, 1985·Regulatory Peptides·J SzecòwkaJ A Williams
Dec 18, 1981·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J D Gardner, R T Jensen
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R T JensenJ D Gardner

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Citations

Oct 17, 1989·European Journal of Pharmacology·S H GutE A Barnard
May 4, 1987·European Journal of Biochemistry·M ZeggariA Ribet
Aug 1, 1993·European Journal of Biochemistry·S Silvente-PoirotD Fourmy
May 1, 1990·Journal of Electron Microscopy Technique·R J WentholdD R Hampson
Aug 31, 1987·Biochemical and Biophysical Research Communications·R K PearsonL J Miller

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