PMID: 7021152Jun 1, 1981Paper

Molecular properties of two mutant species of the elongation factor Tu

European Journal of Biochemistry
P H van der MeideT Blumenthal

Abstract

The molecular properties of two mutant species of the elongation factor Tu (EF-Tu), derived from either tuf A or tuf B, have been studied. One, designated EF-TuAR, is the product of a kirromycin-resistant tufA gene. The other designated EF-TuBO is a tuf B product and is present in a kirromycin-resistant mutant of Escherichia coli (LBE 2012) also harbouring the EF-TuAR species. EF-TuAR has been isolated in homogeneous form as a single gene product from the mutant strain LBE 2045, in which the tuf B gene has been inactivated by an insertion of the bacteriophage Mu. EF-TuBO has been isolated from LBE 2012 together with EF-TuAR in a 1:1 mixture. Fractionation of this mixture of DEAE-Sephadex A-50 resulted in an enrichment of EF-TuBO of about 80%. The properties of EF-TuAR and EF-TuBO have been compared to those of a kirromycin-sensitive species designated EF-TuAS, which was isolated from LBE 2045 by transduction of wild-type tuf A. We show here that all three EF-Tu species are fully competent to sustain polypeptide synthesis. All also appear to interact normally with guanine nucleotides and EF-Ts. Only in the presence of the antibiotic do the following differences appear. (a) Kirromycin causes EF-TuAS (wild-type tuf A gene product)...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E FischerA Parmeggiani
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B D BeckA Jacobson
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A Van de KlundertL Bosch
Sep 21, 1978·Biochimica Et Biophysica Acta·Y Kaziro
Jan 1, 1979·Annual Review of Biochemistry·T Blumenthal, G G Carmichael
Jul 1, 1979·European Journal of Biochemistry·B D Beck
May 6, 1976·Nature·G R Jacobson, J P Rosenbusch
Jun 1, 1976·Biochemistry·G R JacobsonJ P Rosenbusch
Oct 5, 1976·Journal of Molecular Biology·W H GastA Wittinghofer
Nov 16, 1976·Biochemistry·G R Jacobson, J P Rosenbusch
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A V Furano
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H WolfA Parmeggiani
Jul 6, 1973·Nature·A Travers
Sep 1, 1973·Analytical Biochemistry·M W Neal, J R Florini
Jul 1, 1980·European Journal of Biochemistry·A WittinghoferR Leberman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K AraiM Wade
Jul 1, 1980·European Journal of Biochemistry·M D JonesB F Clark
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P H Van der MeideL Bosch
Jun 1, 1981·European Journal of Biochemistry·F J DuisterwinkelL Bosch

❮ Previous
Next ❯

Citations

Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
Jun 1, 1981·European Journal of Biochemistry·F J DuisterwinkelL Bosch
Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
May 1, 1993·Molecular Microbiology·I Tubulekas, D Hughes
May 31, 2003·Nature Reviews. Genetics·Lynn MieselTodd A Black
Mar 10, 1990·European Journal of Biochemistry·S Tapio, L A Isaksson
Dec 5, 1998·Biochimica Et Biophysica Acta·I M Krab, A Parmeggiani
Aug 27, 1990·Biochimica Et Biophysica Acta·J P AbrahamsB Kraal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.