Molecular prophage typing of Staphylococcus aureus isolates from bovine mastitis

Journal of Veterinary Science
Dae-Sung KoHyuk-Joon Kwon

Abstract

Staphylococcus aureus is one of the major pathogens causing bovine mastitis and foodborne diseases associated with dairy products. To determine the genetic relationships between human and bovine or bovine isolates of S. aureus, various molecular methods have been used. Previously we developed an rpoB sequence typing (RSTing) method for molecular differentiation of S. aureus isolates and identification of RpoB-related antibiotic resistance. In this study, we performed spa typing and RSTing with 84 isolates from mastitic cows (22 farms, 72 cows, and 84 udders) and developed a molecular prophage typing (mPPTing) method for molecular epidemiological analysis of bovine mastitis. To compare the results, human isolates from patients (n = 14) and GenBank (n = 166) were used for real and in silico RSTing and mPPTing, respectively. Based on the results, RST10-2 and RST4-1 were the most common rpoB sequence types (RSTs) in cows and humans, respectively, and most isolates from cows and humans clearly differed. Antibiotic resistance-related RSTs were not detected in the cow isolates. A single dominant prophage type and gradual evolution through prophage acquisition were apparent in most of the tested farms. Thus, RSTing and mPPTing are info...Continue Reading

References

Jan 1, 1989·Annual Review of Microbiology·L W Black
Sep 1, 1982·Annals of Internal Medicine·R L ThompsonR P Wenzel
Feb 1, 1995·Journal of Clinical Microbiology·V KapurJ M Musser
Oct 3, 1998·Antimicrobial Agents and Chemotherapy·H Aubry-DamonP Courvalin
Jul 31, 2003·Molecular Microbiology·Sherwood Casjens
Jan 5, 2005·Journal of Bacteriology·James E CassatMark S Smeltzer
Nov 3, 2006·Molecular Microbiology·Taeok BaeOlaf Schneewind
Nov 14, 2006·Annals of Clinical Microbiology and Antimicrobials·Xander W HuijsdensAlbert J de Neeling
Jun 8, 2007·Emerging Infectious Diseases·Eva Juhász-KaszanyitzkyJaap A Wagenaar
Nov 15, 2007·JAMA : the Journal of the American Medical Association·Bridget M Kuehn
Nov 23, 2007·Journal of Clinical Microbiology·B StrommengerW Witte
Feb 9, 2008·Emerging Infectious Diseases·Inge van LooJan Kluytmans
Mar 31, 2009·Journal of Bacteriology·Christiane GoerkeChristiane Wolz
May 12, 2010·Journal of Veterinary Science·Sun Young HwangYong Ho Park
Sep 15, 2010·Antimicrobial Agents and Chemotherapy·Longzhu CuiKeiichi Hiramatsu
Apr 29, 2011·Journal of Clinical Microbiology·Yukiko WatanabeKeiichi Hiramatsu
Jul 12, 2011·Applied and Environmental Microbiology·Olga SakwinskaPhilippe Moreillon
Oct 30, 2012·Veterinary Microbiology·Hyuk-Joon KwonJae-Hong Kim
Nov 13, 2012·Veterinary Microbiology·Stefan MoneckeSonia Cortez de Jäckel
Jan 1, 2013·The Journal of Microbiology·Won-Jin SeongJae-Hong Kim
Nov 10, 2013·The Journal of General and Applied Microbiology·Won-Jin SeongHyuk-Joon Kwon
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

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Methods Mentioned

BETA
PCR

Software Mentioned

ChromasPro
BLAST
RSTing
mPPTing
SPSS
Bioedit
MEGA

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