Abstract
Metastasis involves the spread of cancer cells from the primary tumor to surrounding tissues and distant organs and is the primary cause of cancer morbidity and mortality. The aim of the study was the determination of change in molecular factors expression in primary kidney cancers (ccRCC) and metastatic sites. In total, 62 patients with RCC were enrolled in the study. The mRNA levels of molecular markers were studied by real-time PCR, and the content of the studied parameters was determined by Western blotting and ELISA. The features in the intracellular signal metabolites in the series of normal renal parenchyma, tumor tissue of localized, disseminated kidney cancer and metastatic tissue were studied. A decrease in some indicators in the tissue of the metastatic lesion was noted. Protein products of transcription factors HIF-1, CAIX, PTEN and activated AKT kinase, as well as expression of the VEGFR2 receptor and m-TOR protein kinase were revealed to be reduced in the metastatic sites. In addition, some indicators increased in metastasis: the protein levels of NF-κB p 50, NF-κB p 65, HIF-2, VEGF, VEGFR2, m-TOR and mRNA of HIF-1, CAIX, PTEN and PDK. There were indicators with multidirectional changes. HIF-1, CAIX, PTEN, VEGFR2 ...Continue Reading
References
Jul 24, 2010·Journal of Oncology·Swethajit BiswasAdrian L Harris
Jul 28, 2011·Pathology Oncology Research : POR·Martina HagerChristian Kolbitsch
Mar 9, 2012·The New England Journal of Medicine·Marco GerlingerCharles Swanton
Jun 20, 2012·Critical Reviews in Oncology/hematology·Stéphane VignotJean-Charles Soria
Dec 15, 2012·Critical Reviews in Oncogenesis·Thomas N Seyfried, Leanne C Huysentruyt
Jun 25, 2013·Nature·UNKNOWN Cancer Genome Atlas Research Network
Nov 19, 2013·Annals of Surgical Oncology·Joana HeinzelmannKerstin Junker
Mar 10, 2015·Molecular Cancer·Zhenyi SuQiang Yu
Feb 7, 2016·Urologic Oncology·Aleksandra Semeniuk-WojtaśCezary Szczylik
Nov 26, 2016·Clinical & Experimental Metastasis·B SzekelyJ Kulka
May 11, 2017·Virchows Archiv : an International Journal of Pathology·Julien DagherMarc-Antoine Belaud-Rotureau
Jul 7, 2017·Cancer & Metabolism·Petra MiikkulainenPanu M Jaakkola
Aug 16, 2017·Cancer Immunology Research·Hiroshi KuboBruno Silva-Santos
Aug 17, 2017·International Journal of Molecular Sciences·Ali Mehdi, Yasser Riazalhosseini
Aug 24, 2017·Cancer Medicine·Hiroyuki KitanoAkio Matsubara
Jan 6, 2018·Bulletin of Mathematical Biology·Leonid Hanin, Jason Rose
Feb 7, 2018·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wan-Cai QueChao-Yang Wu
Apr 21, 2018·British Journal of Cancer·Guillermo de VelascoToni K Choueiri
Nov 19, 2018·Critical Reviews in Oncology/hematology·Valentina PolettoCamillo Porta
Mar 27, 2019·Biomolecules·Mazhar PashaShankar Munusamy