Molecular quantification of virulence gene-containing Aeromonas in water samples collected from different drinking water treatment processes.

Environmental Monitoring and Assessment
Chang-Ping Yu, Kung-Hui Chu

Abstract

Pathogenic species of Aeromonas produce a range of virulence factors, including aerolysin, cytotonic enterotoxins, and serine protease, to cause acute gastroenteritis and wound infections in humans and animals. Recognizing that not all Aeromonas strains are pathogenic, in this study, we proposed to evaluate Aeromonas removal effectiveness based on the presence of virulence gene-containing Aeromonas as a proper means to assess microbial risk of Aeromonas. We developed and applied real-time PCR assays to quantify serine protease (ser) gene- and heat-labile cytotonic enterotoxin (alt) gene-containing Aeromonas in water samples. Among 18 Aeromonas isolates from the source water, only three isolates possessed all three genes (aer, ser, and alt). A higher percent of isolates has either ser gene (89%) or alt gene (72%) compared to the percent of isolates containing aer gene (44%). Results of this study suggested that several different conventional and unconventional drinking water treatment processes could effectively remove Aeromonas from source water. As the comprehensive knowledge of the distribution of virulence factors in different Aeromonas species is currently not available, using real-time PCR to quantify various virulence fac...Continue Reading

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Feb 15, 2011·International Journal of Environmental Research and Public Health·M José Figueras, Juan J Borrego
Mar 7, 2017·Environmental Science. Processes & Impacts·Hassan WaseemSyed A Hashsham
Apr 10, 2020·PloS One·Kyle D BrumfieldAnwar Huq

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