PMID: 7544796Sep 1, 1995Paper

Molecular regulation of GLUT-4 targeting in 3T3-L1 adipocytes

The Journal of Cell Biology
B J MarshD E James

Abstract

Insulin stimulates glucose transport in muscle and adipose tissue by triggering the movement of the glucose transporter GLUT-4 from an intracellular compartment to the cell surface. Fundamental to this process is the intracellular sequestration of GLUT-4 in nonstimulated cells. Two distinct targeting motifs in the amino and carboxy termini of GLUT-4 have been previously identified by expressing chimeras comprised of portions of GLUT-4 and GLUT-1, a transporter isoform that is constitutively targeted to the cell surface, in heterologous cells. These motifs-FQQI in the NH2 terminus and LL in the COOH terminus-resemble endocytic signals that have been described in other proteins. In the present study we have investigated the roles of these motifs in GLUT-4 targeting in insulin-sensitive cells. Epitope-tagged GLUT-4 constructs engineered to differentiate between endogenous and transfected GLUT-4 were stably expressed in 3T3-L1 adipocytes. Targeting was assessed in cells incubated in the presence or absence of insulin by subcellular fractionation. The targeting of epitope-tagged GLUT-4 was indistinguishable from endogenous GLUT-4. Mutation of the FQQI motif (F5 to A5) caused GLUT-4 to constitutively accumulate at the cell surface re...Continue Reading

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Citations

Feb 27, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Baumann, A R Saltiel
Apr 1, 1999·Cell Biochemistry and Biophysics·S MartinD E James
Apr 18, 2001·Trends in Cell Biology·G D Holman, I V Sandoval
Feb 19, 2002·Trends in Cell Biology·Alan R Saltiel, Jeffrey E Pessin
Jun 14, 2008·Molecular Biology of the Cell·Vincent Blot, Timothy E McGraw
May 26, 2001·The Journal of Clinical Investigation·M Mueckler
Nov 24, 2011·The Journal of Endocrinology·Valerie M HarrisColleen Buggs-Saxton
May 19, 1997·The Journal of Cell Biology·M KovalT H Steinberg
Jul 11, 2006·Biochemical and Biophysical Research Communications·Kirsty M ProctorGwyn W Gould
Apr 5, 2000·Biochimica Et Biophysica Acta·B G Forde
Jan 23, 1999·The Journal of Biological Chemistry·J E PessinS Okada
Jun 28, 2020·Pflügers Archiv : European journal of physiology·Geoffrey D Holman
Sep 2, 1998·The American Journal of Physiology·B J MarshD E James
Oct 19, 1999·Molecular and Cellular Biology·M M HillS L Macaulay
Oct 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Su BuFuliang Cao

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