Molecular replacement studies on crystal structure of allophycocyanin from red algaePorphyra yezoensis

Science in China. Series C, Life Sciences
J LiuD C Liang

Abstract

Using the crystal structure of allophycocyanin from cyanobacteriumSpirulina platensis (APC-SP) as a search model, the crystal structure of allophycocyanin from red algaePorphyra yezoensis (APC-PY) has been studied by molecular replacement methods. The APC-PY crystals (Form 3) belong to the space group of R32, cell dimensions a =b = 10.53 nm,c = 18.94 nm, alpha= beta = 90 degrees , gamma= 120 degrees ; there is one alphabeta monomer in each crystallographic asymmetric unit in the cell. The translation function search gave a unique peak with a correlation coefficient (Cc) of 67.0% and an R-factor of 36.1 % for reflection data from 1.0 to 0.4 nm. Using the results by molecular replacement, the initial model of APC-PY was built, and the coincidence of the chromophore in APC-PY initial model with its2F (o)- F(c) OMIT map further confirms the results by molecular replacement.

References

Oct 11, 1996·Journal of Molecular Biology·W R ChangD C Liang
Oct 8, 1998·Acta Crystallographica. Section D, Biological Crystallography·J Y LiuH J Wu

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Citations

Mar 2, 2018·Journal of Inequalities and Applications·Imran Nadeem, Hani Shaker

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