Molecular serogrouping of Escherichia coli.

Animal Health Research Reviews
Chitrita DebRoyElisabeth Roberts

Abstract

O-antigens present on the surface of Escherichia coli provide antigenic specificity for the strain and are the main components for O-serogroup designation. Serotyping using O-group-specific antisera for the identification of E. coli O-serogroups has been traditionally the gold-standard for distinguishing E. coli strains. Knowledge of the O-group is important for determining pathogenic lineage, classifying E. coli for epidemiological studies, for determining virulence, and for tracing outbreaks of diseases and sources of infection. However, serotyping has limitations, as the antisera generated against each specific O-group may cross-react, many strains are non-typeable, and others can autoagglutinate or be rough (lacking an O-antigen). Currently, the nucleotide sequences are available for most of the 187 designated E. coli O-groups. Public health and other laboratories are considering whole genome sequencing to develop genotypic methods to determine O-groups. These procedures require instrumentation and analysis that may not be accessible and may be cost-prohibitive at this time. In this review, we have identified unique gene sequences within the O-antigen gene clusters and have targeted these genes for identification of O-group...Continue Reading

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Citations

Jul 7, 2020·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (EFSA BIOHAZ Panel)Lieve Herman
Feb 9, 2021·Ecotoxicology and Environmental Safety·Qian TangChunmei Li

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