Molecular Sex Identification in the Hardy Rubber Tree (Eucommia ulmoides Oliver) via ddRAD Markers

International Journal of Genomics
Wencai WangXianzhi Zhang

Abstract

Eucommia ulmoides, also known as the industrially and medicinally important hardy rubber tree, is the sole species of Eucommiaceae. Nevertheless, its dioecious property hinders sex recognition by traditional morphological observation at very early developmental stages, thus inhibiting breeding and economic cropping. In this study, double-digest restriction site-associated DNA sequencing (ddRAD-seq) was applied to screen sex-linked molecular markers for sex identification and investigation of the sex determination system in 20 male and female E. ulmoides individual plants, respectively. In consequence, five candidate male-specific loci but no female-specific loci were predicated among the 183,752 male and 147,122 female catalogue loci by bioinformatics analysis. Subsequent PCR (polymerase chain reaction) amplification and Sanger sequencing examinations were performed on another 24 individuals, 12 for each sex, from a separate population. One ideal sex-linked locus, MSL4, was identified among the five putative male-specific loci that were found using ddRAD data. MSL4 is 479 bp in length and highly conserved in all the male individuals, suggesting its feature of being stable and repeatable. Our results also indicated that the sex ...Continue Reading

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Citations

Mar 25, 2021·Journal of Agricultural and Food Chemistry·Xingneng WeiJuane Dong
Jul 25, 2020·Scientific Reports·Xiao FengWang Ying

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Datasets Mentioned

BETA
PRJNA607161

Methods Mentioned

BETA
Illumina Sequencing
PCR
ddRAD-seq
electrophoresis

Software Mentioned

sstacks
Cutadapt
Linux
cstacks
R script
FastQC
Geneious
Stacks
Primer3

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