Molecular-shape imprinting and immobilization of biomolecules on a polymer containing azo dye

Langmuir : the ACS Journal of Surfaces and Colloids
Taiji IkawaOsamu Watanabe

Abstract

We demonstrate a novel technique for molecular imprinting and immobilization on a surface of a polymer containing azo dyes (azopolymer). The azopolymer was found to be capable of immobilizing micrometer- and nanometer-scale macromolecules (e.g., lambda-DNA, immunoglobulin G (IgG), bacterial protease, and 1-mum polystyrene particles) through photoirradiation with blue-wavelength light. Fluorescence and atomic force microscopy studies revealed that the azopolymer surface deformed along with the shape of the macromolecules, holding them in place after photoirradiation. The desorption of the immobilized macromolecules from the azopolymer surface in an aqueous medium was observed to be very slow, on the time scale of 10 min to weeks, depending on the photoirradiation time. Immunological and enzymatic studies showed that IgG and bacterial protease immobilized on the azopolymer surface retained their original functionality. These results suggest that the azopolymer physically, not chemically, binds the macromolecules because of the increase in contact area between the macromolecules and the azopolymer surface after photoirradiation.

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Citations

Nov 10, 2006·The Journal of Chemical Physics·Maria Sabaye Moghaddam, Hue Sun Chan
Nov 21, 2014·Journal of Clinical Biochemistry and Nutrition·Koufuchi RyoIchiro Saito
Nov 26, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Tao WeiKatherine Shing
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Dec 7, 2010·Chemical Society Reviews·Michael J WhitcombeAdrian Horgan
Nov 9, 2017·Chemistry : a European Journal·Philipp WeisSi Wu
Mar 16, 2007·Physical Review Letters·Taiji IkawaCyrus R Safinya
Jul 8, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Taiji IkawaHiroshi Shimoyama
Jan 23, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·H WatanabeK Fujimoto
Oct 30, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Hao HuangXiaogong Wang

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