PMID: 7538141May 1, 1995Paper

Molecular size-fractionation during endocytosis in macrophages

The Journal of Cell Biology
E P BerthiaumeJ A Swanson

Abstract

The sorting of macromolecules within and between membranous organelles is often directed by information contained in protein primary or secondary structure. We show here that absent such structural information, macromolecules internalized by endocytosis in macrophages can be sorted by size. After endocytosis, small solute probes of fluid-phase pinocytosis were recycled to the extracellular medium more efficiently than large solutes. Using macropinosomes pulse labeled with fluorescent dextrans, we examined the ability of organelles to exchange solute contents. Dextran exchange was optimal between organelles of similar age, and small dextrans exchanged more efficiently than large dextrans. Efferent solute movement, from lysosomes or phagolysosomes toward the plasma membrane, occurred through the same endocytic vesicles as afferent movement, toward lysosomes and this movement was solute size dependent. Remarkably, uniform mixtures of different-sized dextrans delivered into lysosomes separated into distinct organelles containing only one dextran or the other. Thus, the dynamics of endosomes and lysosomes were sufficient to segregate macromolecules by size. This intracellular size fractionation could explain how, during antigen pres...Continue Reading

References

Aug 1, 1978·The Journal of Cell Biology·M C Willingham, S S Yamada
Mar 1, 1976·The Journal of Cell Biology·R M SteinmanZ A Cohn
Aug 30, 1990·Nature·P J Hollenbeck, J A Swanson
Jan 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z R Luo, J M Robinson
Jan 30, 1992·Nature·J E Rothman, L Orci
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C V HardingH J Geuze
Dec 1, 1987·The Journal of Cell Biology·A L FerrisB Storrie
Nov 1, 1989·The Journal of Experimental Medicine·E L Racoosin, J A Swanson
Sep 1, 1987·The Journal of Cell Biology·R Matteoni, T E Kreis
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SwansonS C Silverstein
Jun 1, 1993·The Journal of Cell Biology·E L Racoosin, J A Swanson
Mar 1, 1994·The Journal of Cell Biology·L J HewlettC Watts

❮ Previous
Next ❯

Citations

Aug 5, 1996·Biotechnology and Bioengineering·A R French, D A Lauffenburger
Jul 9, 1999·Reviews in Medical Virology·N E Bishop
Nov 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B Storrie, M Desjardins
Dec 24, 1997·Journal of Magnetic Resonance Imaging : JMRI·A MooreA Bogdanov
May 26, 2001·Bulletin of Mathematical Biology·C A HeckmanS Seubert
Jul 1, 1997·Annals of Biomedical Engineering·A R French, D A Lauffenburger
Apr 27, 2012·Inflammopharmacology·G Joseph BroussardGloria J Klapstein
Aug 1, 1995·Current Opinion in Cell Biology·J Gruenberg, F R Maxfield
Jul 1, 1997·Molecular and Biochemical Parasitology·I D GoodyerT F Taraschi
Dec 5, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·M HaradaT Suzuki
Mar 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·M HaradaT Suzuki
Feb 1, 1997·Current Opinion in Immunology·J Pieters
Nov 1, 1995·Trends in Cell Biology·J A Swanson, C Watts
Apr 22, 2010·Organic Letters·Michael D PluthStephen J Lippard
Sep 21, 2006·Nature Immunology·J Magarian Blander, Ruslan Medzhitov
Feb 24, 2001·Cellular Microbiology·S Duclos, M Desjardins
Dec 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bahram KasmapourMaximiliano Gabriel Gutierrez
Feb 1, 1996·The Journal of Cell Biology·Y K Oh, J A Swanson
Jun 7, 2013·Molecular Biology of the Cell·Andrew W WeflenRamiro H Massol
Sep 26, 1997·The Journal of Eukaryotic Microbiology·T L SteckS Meredith
Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
Apr 15, 1999·Annual Review of Neuroscience·F González-Scarano, G Baltuch
Apr 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shweta MandrekarGary E Landreth
Jan 5, 2014·Nature Protocols·Cosimo CommissoDafna Bar-Sagi
May 22, 2008·Expert Opinion on Drug Delivery·María J VicentAntonio Pineda-Lucena
May 5, 2007·The Journal of Physiology·Haibing TengRobert S Wilkinson
Sep 22, 2009·Journal of Neuroscience Methods·Emma Louise Clayton, Michael Alan Cousin
Mar 10, 2009·International Immunopharmacology·Marina A DergunovaTatyana A Korolenko
May 2, 2008·Fish & Shellfish Immunology·Tom Christian TonheimRoy Ambli Dalmo
Jan 11, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·F Philipp SeibRuth Duncan
Sep 1, 2007·Journal of Cellular and Molecular Medicine·Arwyn Tomos Jones
Jun 20, 2007·Electrophoresis·Yun ChenEdgar A Arriaga
Feb 6, 2007·Traffic·Albert Haas
Apr 9, 2008·Biotechnology and Bioengineering·Anne GigoutMario Jolicoeur
Jun 17, 2008·Biotechnology and Bioengineering·D Ece GamsizRebecca L Carrier
Oct 7, 2015·The Journal of Cell Biology·Sei YoshidaJoel A Swanson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Heather P McDowellCarlo Dominici
© 2021 Meta ULC. All rights reserved