Jan 25, 1976

Molecular size of nerve growth factor in dilute solution

The Journal of Biological Chemistry
M YoungB G Arnason

Abstract

Nerve growth factor (NGF) is a protein composed of two identical chains of mass 13,259. An analysis of the sedimentation equilibrium, sedimentation velocity, and gel filtration behavior of dilute solutions of NGF indicates the existence of a rapidly reversible monomer in equilibrium dimer equilibrium and that the association constant K for the reaction at neutral pH is 9.4 X 10(6)M-1. Reaction mixtures consist of equal concentrations of monomer and dimer at a total protein concentration as high as 1.4 mug/ml, and at 1 ng/ml, monomer accounts for greater than 99% of the total. The latter concentration is 20 to 30 times that required for the biological activity of NGF. Several lines of evidence suggest that the dimerization reaction is highly stereospecific, although its biological significance is not known.

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Mentioned in this Paper

Nerve Growth Factor
Neurite Outgrowth Factors
Gel Chromatography
Spectrophotometry, Ultraviolet
Recombinant Nerve Growth Factor
Chick Embryo
Dimer
Sedimentation Procedure
NGF gene
Protein Concentration, Test Strip Measurement

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