Molecular species determination of oligosaccharides and glycoconjugates in soybean lecithin powders

Journal of the Science of Food and Agriculture
Jingjing KangDong Cao

Abstract

Oligosaccharides and glycoconjugates in soybean lecithin powder are very important for the properties and functions of the materials that contain it. Oligosaccharides can trigger infusion reactions and color changes in soybean lecithin during medical injections; glycoconjugates in the form of glycolipids can also change the physical behavior of lecithin. The molecular components of oligosaccharides and glycoconjugates in soybean lecithin powder were studied in this paper. Three oligosaccharides and 21 glycoconjugates were verified in soybean lecithin powders for the first time. Raffinose, sucrose, and stachyose were detected as the principal components of oligosaccharides by high-performance liquid chromatography (HPLC). Twenty-one glycoconjugates, including four steryl glucosides (SG), 13 acyl steryl glucosides (ASG), one digalactosyl diacylglycerol (DGDG), one monogalactosyl diacylglyceride (MGDG), one glucocerebroside (Glucer), and one glucose palmitate were analyzed by gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and RP-UPLC-Q-TOF-ESI-MS. Glycoconjugates were constructed in soybean lecithin powder after separation by column chromatography, thin-layer chromatography, and color reactions. The determin...Continue Reading

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