Molecular spectroscopic studies on the interaction of morin with bovine serum albumin

Journal of Photochemistry and Photobiology. B, Biology
Yan-Jun HuLi-Ping Zhang

Abstract

The interaction between morin and bovine serum albumin (BSA) was studied using molecular spectroscopic approach at different temperatures under imitated physiological conditions. Quenching of intrinsic tryptophanyl fluorescence of BSA with increasing morin concentration is the actuating tool in the analysis. The obtained quenching mechanisms, binding constants, binding sites and corresponding thermodynamic parameters at different temperatures indicate that the hydrophobic interaction play a major role in the morin-BSA association. Binding affinity between morin and BSA was determined using Scatchard equation and the modified Stern-Volmer equation, and the corresponding Structure-affinity relationships of flavonoids were discussed. Site marker competitive displacement experiments demonstrated that morin binds with high affinity to site II (subdomain IIIA) of BSA. Furthermore, the circular dichroism spectral results indicated that the conformation of BSA changed in the presence of morin. In addition, the effect of some common metal ions on the binding constant between morin and BSA was examined.

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Citations

Sep 5, 2013·Journal of Photochemistry and Photobiology. B, Biology·Bingjun YangRutao Liu
Feb 26, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Chengbin XuXuelian Meng
Feb 12, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ali DadrasFarzad Mokhtari
Oct 11, 2013·Luminescence : the Journal of Biological and Chemical Luminescence·Hongmei ZhangYanqing Wang
Jan 30, 2014·Biochimica Et Biophysica Acta·Yury S TarahovskyEugeny N Muzafarov
Jan 27, 2015·Journal of Photochemistry and Photobiology. B, Biology·Fang HaoRutao Liu
May 20, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Nahid ShahabadiSoraya Moradi Fili
Aug 3, 2019·Journal of Molecular Recognition : JMR·Tongtong ZhengChunguang Liu
Feb 20, 2020·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Jie BaiXuekai Sun
Jan 13, 2018·Scientific Reports·Guoqing XuRunguang Sun

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