PMID: 7515202Jun 1, 1994Paper

Molecular staging of prostate cancer with the use of an enhanced reverse transcriptase-PCR assay

Urology
A E KatzR Buttyan

Abstract

Because up to 40 percent of surgically treated patients with prostate cancer are subsequently found to be clinically understaged, a more sensitive staging modality to identify extraprostatic disease prior to surgery is required. We describe an enhanced reverse transcriptase [RT] polymerase chain reaction (PCR) assay utilizing oligonucleotide primers specific for the human prostate-specific antigen (PSA). This assay identifies PSA-synthesizing cells from reverse transcribed mRNA. This assay was applied to RNAs extracted from the peripheral blood lymphocytes of 65 patients with clinically localized prostate cancer. In addition, blood from 20 women, 20 young men, 25 age-matched control men under treatment for benign prostatic hyperplasia (BPH), and 18 men with established, untreated metastatic prostate cancer was tested. An RT-PCR assay for PSA can recognize one PSA-expressing cell diluted into one hundred thousand lymphocytes. The sensitivity of this assay can be enhanced by the addition of digoxigenin-modified nucleotides to the PCR reaction and this assay was applied to RNAs extracted from the peripheral lymphocyte fraction of 148 prostate cancer patients and controls at this institution. Although no specimen from women or men ...Continue Reading

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Citations

Jun 1, 1997·The Prostate·X GaoK V Honn
Jan 6, 1997·International Journal of Cancer. Journal International Du Cancer·W HenkeS A Loening
Aug 4, 1998·International Journal of Cancer. Journal International Du Cancer·E Corey, M J Corey
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Mar 9, 2007·World Journal of Urology·Thomas SteuberHans Lilja
Sep 4, 2008·Current Urology Reports·Stephan JeschkeUrs E Studer
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Jul 4, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·J A López-GuerreroS Bonanad-Boix
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Nov 1, 1996·Steroids·M E Lombardo, P B Hudson
Jun 1, 1996·Urology·A E KatzR Buttyan
Mar 1, 1997·Urology·P R CarrollM Roach

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