Molecular Strings Significantly Improved the Gene Transfection Efficiency of Polycations

Journal of the American Chemical Society
Huapan FangXuesi Chen

Abstract

High transfection efficiency and low cytotoxicity are the two key factors to be considered in the design of gene carriers. Herein, a novel and versatile gene carrier (PLL-RT) was prepared by introducing "molecular string" RT (i.e., p-toluylsulfonyl arginine) onto the polylysine backbone. The introduction of RT string contributed to the formation of multiple interactions between the polycationic gene carriers and cell membrane or DNA, as well as adopting α-helix conformation, all of which would be beneficial to enhance the gene transfection. In addition, RT string grafted onto other polycations such as hyperbranced PEI25k and dendrimer PAMAM could also acquire improved transfection efficiency and low cytotoxicity. Moreover, PLL-RT presented significant tumor inhibition effect in vivo. This work provided an effective strategy for constructing novel gene carriers with high transfection and low cytotoxicity.

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Citations

Apr 3, 2019·The Journal of Gene Medicine·Jiayan WuXuesi Chen
Sep 5, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bo LiuXiaoyan Yuan
Dec 4, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Janelle W SalamehMarxa L Figueiredo
Jun 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang ZhouJun Wu
Jun 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chenglong GeLichen Yin
Jun 18, 2020·International Journal of Nanomedicine·Rajasekharreddy PalaSurya M Nauli
Oct 7, 2020·Biomaterials Science·Xiaojing ChenYilong Cheng
Oct 8, 2020·Biomaterials Science·Kübra Kaygisiz, Christopher V Synatschke
Dec 18, 2020·Advanced Drug Delivery Reviews·Yong Liu, Lichen Yin
Oct 30, 2019·Biomacromolecules·Huanli SunChao Deng
Nov 2, 2018·Bioconjugate Chemistry·Jie ChenXuesi Chen
May 3, 2019·Bioconjugate Chemistry·Yingqin Hou, Hua Lu

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