Molecular structure and developmental expression of three muscle-type troponin T genes in zebrafish

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Chung-Der HsiaoHuai-Jen Tsai

Abstract

Troponin T (Tnnt), a troponin component, interacts with tropomyosin and is crucial to the regulation of striated muscle contraction. To gain insight into the molecular evolution and developmental regulation of Tnnt gene (Tnnt) in lower vertebrates, zebrafish Tnnt1 (slow Tnnt), Tnnt2 (cardiac Tnnt), and Tnnt3b (fast Tnnt isoform b) were characterized. The polypeptides of zebrafish Tnnt1, Tnnt2, and Tnnt3b were conserved in the central tropomyosin- and C-terminal troponin I-binding domains. However, the N-terminal hypervariable regions were highly extended and rich in glutamic acid in polypeptides of Tnnt1 and Tnnt2, but not Tnnt3b. The Tnnt2 and Tnnt3b contain introns, whereas Tnnt1 is intron-free. During development, large to small, alternatively spliced variants were detected in Tnnt2, but not in Tnnt1 or Tnnt3. Whole-mount in situ hybridization showed zebrafish Tnnt1 and Tnnt2 are activated during early somitogenesis (10 hr postfertilization, hpf) and cardiogenesis (14 hpf), respectively, but Tnnt3b is not activated until middle somitogenesis (18 hpf). Tnnt2 and Tnnt3b expression was cardiac- and fast-muscle specific, but Tnnt1 was expressed in both slow and fast muscles. We propose that three, distinct, muscle-type Tnnt evol...Continue Reading

References

Mar 1, 1991·General and Comparative Endocrinology·K YamanoY Inui
Oct 27, 1995·Gene·J FitzgibbonD M Hunt
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S AparicioS Brenner
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Oct 1, 1996·Journal of Biochemistry·A InoueK Nishita
Feb 1, 1997·Cell Structure and Function·F E Stockdale
Apr 25, 1997·Journal of Molecular Biology·C Burge, S Karlin
Apr 16, 1998·Nature Genetics·J H PostlethwaitW S Talbot
Aug 5, 1998·Journal of Molecular and Cellular Cardiology·H FarzaK Schwartz
Sep 22, 1998·Journal of Muscle Research and Cell Motility·S V Perry
Jun 11, 1999·The Journal of Biological Chemistry·E A BucherC P Emerson
Jun 16, 1999·Molecular Biology and Evolution·W R PearsonT Zhang
Jun 16, 1999·Molecular Biology and Evolution·S OOta, N Saitou
Sep 3, 1999·Journal of Muscle Research and Cell Motility·D M WaddletonD H Heeley
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·J H MardenB Rowan
Jan 5, 2000·The Journal of Experimental Zoology·I YonemuraJ Miyazaki
Jul 6, 2000·FEBS Letters·B VenkateshS Brenner
Sep 26, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y XuZ Gong
Feb 24, 2001·Journal of Muscle Research and Cell Motility·K KrishanG K Dhoot

❮ Previous
Next ❯

Citations

May 16, 2013·Molecular Biotechnology·Noriko UmemotoToshio Tanaka
Dec 20, 2013·Assay and Drug Development Technologies·Cheng-Chen HuangKarl P Peterson
Jul 24, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Fred Tilton, Robert L Tanguay
Jun 20, 2007·BMC Developmental Biology·Marco A CampinhoDeborah M Power
Feb 10, 2011·Disease Models & Mechanisms·Laura E Machuca-TziliJ David Brook
Jan 20, 2011·Journal of Cell Science·Maria I FerranteDerek L Stemple
Nov 23, 2006·Zoological Science·Yukihiko YamasakiToru Higashinakagawa
Oct 15, 2013·Environmental Toxicology and Chemistry·Amy BeasleyMatthew Elrod-Erickson
Jul 31, 2012·Molecular and Cellular Endocrinology·Dinushan Nesan, Mathilakath M Vijayan
May 1, 2009·Journal of Fish Biology·S-W ChongY-J Jiang
Jul 31, 2008·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Pierre-Yves Rescan
Sep 20, 2011·Molecular Ecology·Andrew R WhiteleyLouis Bernatchez
Jun 16, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A C BurguièreJ von Hofsten
Sep 1, 2006·Mechanisms of Development·Yuexiang WangHouyan Song
Dec 2, 2004·Developmental Biology·Anskar Y H LeungCatherine M Verfaillie
Dec 4, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chung-Der HsiaoHuai-Jen Tsai
Dec 1, 2017·Scientific Reports·Toshihiro KawasakiNoriyoshi Sakai
Jul 2, 2019·Frontiers in Endocrinology·Marco António Campinho
Apr 4, 2021·International Journal of Molecular Sciences·Stephanie L PadulaKatherine E Yutzey
Oct 23, 2010·Archives of Biochemistry and Biophysics·Bin Wei, J-P Jin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.