Molecular structure and target recognition of neuronal calcium sensor proteins.

Frontiers in Molecular Neuroscience
James B AmesMitsuhiko Ikura

Abstract

Neuronal calcium sensor (NCS) proteins, a sub-branch of the EF-hand superfamily, are expressed in the brain and retina where they transduce calcium signals and are genetically linked to degenerative diseases. The amino acid sequences of NCS proteins are highly conserved but their physiological functions are quite distinct. Retinal recoverin and guanylate cyclase activating proteins (GCAPs) both serve as calcium sensors in retinal rod cells, neuronal frequenin (NCS1) modulates synaptic activity and neuronal secretion, K(+) channel interacting proteins (KChIPs) regulate ion channels to control neuronal excitability, and DREAM (KChIP3) is a transcriptional repressor that regulates neuronal gene expression. Here we review the molecular structures of myristoylated forms of NCS1, recoverin, and GCAP1 that all look very different, suggesting that the sequestered myristoyl group helps to refold these highly homologous proteins into very different structures. The molecular structure of NCS target complexes have been solved for recoverin bound to rhodopsin kinase (RK), NCS-1 bound to phosphatidylinositol 4-kinase, and KChIP1 bound to A-type K(+) channels. We propose that N-terminal myristoylation is critical for shaping each NCS family m...Continue Reading

Citations

Dec 27, 2015·Colloids and Surfaces. B, Biointerfaces·Samuel LemireÉlodie Boisselier
Jan 30, 2015·Protein Science : a Publication of the Protein Society·Khoa PhamJaroslava Miksovska
May 24, 2015·Biochimica Et Biophysica Acta·Evgeni Yu ZerniiKarl-W Koch
Dec 3, 2014·Biochimica Et Biophysica Acta·Robert D Burgoyne, Lee P Haynes
Mar 17, 2015·Free Radical Biology & Medicine·Evgeni Yu ZerniiSergei E Permyakov
Mar 24, 2015·American Journal of Human Genetics·Gavin CharlesworthNicholas W Wood
May 23, 2015·Biochemical and Biophysical Research Communications·Christopher B MarshallMitsuhiko Ikura
Sep 10, 2013·Structure·Pétur O HeidarssonCiro Cecconi
Nov 16, 2013·PloS One·Sunghyuk LimJames B Ames
Nov 13, 2010·Biochimica Et Biophysica Acta·Malgorzata PalczewskaJose R Naranjo
Mar 8, 2012·Molecular Neurobiology·Jeffrey S DasonAlberto Ferrús
Sep 29, 2012·The Journal of Biological Chemistry·Pradeep L RamachandranRajiv Kumar
Jun 3, 2015·The Journal of Biological Chemistry·Kristoff T HomanJohn J G Tesmer

❮ Previous
Next ❯

Methods Mentioned

BETA
myristoylation
nuclear magnetic resonance
NMR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.